玻连蛋白促进肝癌细胞株增殖及迁移的初步研究  

A pilot study on the proliferation and migration enhancing effects of vitronectin in SMMC 7721 cells

作　　者：梁晓芸[1] 李文学[1] 陈建玲[2] 李军涛[1] 吴锦银[1] 杨光宇[1] 朱伟[1] 

机构地区：[1]广州市疾病预防控制中心,广东广州510440 [2]广东省职业病防治院,广东广州510300

出　　处：《癌变．畸变．突变》2014年第1期30-34,39,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金青年项目(81101562);广东省自然科学基金面上项目(S2012010009633)

摘　　要：目的:了解玻连蛋白(VTN)对肝肿瘤细胞株SMMC 7 721增殖和迁移能力的影响。方法:采用不同浓度VTN包被培养皿后接种、培养细胞,采用激光共聚焦显微镜观察VTN包被后细胞骨架成分微管蛋白形态的变化,用细胞增殖试剂分析VTN对细胞增殖率的影响及分析VTN对凋亡诱导剂抑制增殖作用的影响,采用Transwell侵袭实验检测VTN对细胞迁移能力的影响。结果:激光共聚焦观察结果显示VTN包被有助于促进细胞贴壁及细胞骨架蛋白形态结构的维持;VTN可促进肿瘤细胞的生长且呈剂量效应,并可减弱凋亡诱导剂对细胞增殖的抑制;Transwell侵袭实验结果显示VTN包被可促进细胞迁移。结论:体外实验条件下,VTN有助于肝癌细胞株SMMC 7 721的增殖及迁移,提示VTN在肝癌发生过程中可能具有促进肿瘤细胞增殖和迁移的生物学作用。OBJECTIVE：To understand the effects of vitronectin （VTN） on cell proliferation and migration in the hepatocellular carcinoma cell line SMMC 7721. METHODS：SMMC 7721 cells were cultured in VTN-coating Petri dishes. The morphology of the cells and the morphology ofβ-tubulin were studied with confocal microscope. WST-1 was used to assess the effect of VTN on cell proliferation rate and the protective effects from the stimuli of apoptosis-inducer. The effect on migration was evaluated with transwell chamber. RESULTS：VTN helped cells adhere to the Petri dishes as well as sustaining the morphology of β-tubulin. VTN could enhance the proliferation rate of SMMC 7721 in a dose-effect mode. VTN could protect the cells from the stimuli of apoptosis-inducer. VTN could also help cell migration across the membrane of transwell chamber. CONCLUSION：VTN might play important roles in the following biological effects,such as sustaining the morphology of the cytoskeleton,promoting adherence of tumor cells,enhancing proliferation,protecting cells from the stimuli of apoptosis-inducer as well as enhancing their migration.

关 键 词：玻连蛋白 肝细胞癌 细胞增殖 肿瘤细胞迁移 

分 类 号：R977.21[医药卫生—药品]