聚乳酸防粘连膜对晚期内皮祖细胞功能的影响  

The effects of the PLLA adhesion prevention membrane on the functions of late endothelial progenitor cells

作　　者：尹青令[1] 侯宁宁[1] 崔晓栋[2] 张爱华[1] 孙金隆[2] 靖旭[2] 吴海燕[2] 王济潍[3] 成敏[2] 

机构地区：[1]潍坊医学院附属医院内分泌科,山东潍坊261053 [2]潍坊医学院医学研究实验中心,山东潍坊261053 [3]山东大学医学院,济南250012

出　　处：《功能材料》2014年第3期101-105,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(30900290;31270993);国家教育部"新世纪优秀人才计划"资助项目(NCET-10-0922)

摘　　要：聚乳酸(poly L-lactic acid,PLLA)及其共聚物的物理和化学性能俱佳,具有可降解性和生物相容性。以晚期内皮祖细胞(endothelial progenitor cells,EPCs)为研究对象,分别运用CCK-8检测法、粘附能力测定实验、Boyden小室迁移实验、Matrigel胶成血管实验、流式细胞仪检测法、细胞衰老特异性β-半乳糖苷酶染色等方法研究聚乳酸防粘连膜对晚期EPCs功能的影响。结果显示,将晚期EPCs种植于聚乳酸防粘连膜上培养一定时间后,细胞的增殖、粘附、迁移、成血管功能均降低,衰老比率增高,而凋亡未见明显差异。该研究结果提示聚乳酸防粘连膜可通过抑制晚期EPCs的功能来预防感染或手术后永久性粘连的发生。To investigate the effects of the PLLA adhesion prevention membrane on the functions of late endothe-lial progenitor cells （EPCs）.The 3 rd generation EPCs,namely late EPCs,was harvested and cell biological functions,such as proliferation,adhesion,migration,the ability of forming tubes,apoptosis and senescence were assayed with CCK-8,adhesion test,Boyden chamber assay,Matrigel,flow cytometry andβ-Galactosidase staining,respectively.The late EPCs implanted on the PLLA adhesions prevention membrane exhibited de-creased capabilities of proliferation,adhesion,migration and tube formation.In the mean time,PLLA increased the senescence of late EPCs but had no effect on cell apoptosis.Therefore,PLLA inhibits late EPC functions, which may be one of the mechanisms that PLLA adhesion prevention membrane prevents permanent adhesions.

关 键 词：聚乳酸防粘连膜 内皮祖细胞 预防粘连 粘附 成血管 

分 类 号：R318[医药卫生—生物医学工程]