高效液相色谱法测定稻田中丙草胺残留  

Determination of Pretilachlor in Paddy Field by HPLC

作　　者：罗俊凯[1] 黄雪[1] 杨丽华[1] 廖晔颉 龚道新[1,2] 

机构地区：[1]湖南农业大学资源环境学院,湖南长沙410128 [2]湖南农业大学农业环境保护研究所,湖南长沙410128

出　　处：《环境监测管理与技术》2014年第1期32-34,共3页The Administration and Technique of Environmental Monitoring

基　　金：农业部农药残留研究基金资助项目(2010H218);广西省南宁市专利新产品产业化重大科技专项基金资助项目(201106055D)

摘　　要：用二氯甲烷提取稻田水和水稻植株样品中的丙草胺,稻田土壤样品用二氯甲烷-甲醇混合溶剂(体积比为9∶1)提取,再用高效液相色谱仪测定。方法在0.02 mg/L^2.00 mg/L的范围内线性良好,相关系数为0.999 8;稻田水、土壤,水稻植株中丙草胺的检出限分别为0.001 mg/L、0.005 mg/kg、0.01 mg/kg;对稻田水、土壤和水稻植株分别做3个浓度水平的加标回收试验,重复5次,平均回收率在75.5%~84.7%之间,测定结果的相对标准偏差为2.0%~10.5%。Pretilachlor in paddy-field water and paddy rice samples were extracted with dichloromethane, while paddy-field soil samples were extracted with dichloromethane-methanol mixture（volume ratio is 9∶1 ）.The extracts were subsequently determined by high performance liquid chromatography.The results showed that the linearity of pretilachlor was favorable in a range between 0.02 mg/L and 2.00 mg/L,the correlation coefficient was 0.999 8.The detection limits of pretilachlor in paddy-field water,paddy-field soil and paddy rice were 0.001 mg/L,0.005 mg/kg and 0.01 mg/kg,respectively.The spiked recovery test of three concentration levels were done in paddy-field water,paddy-field soil and paddy rice,and repeated five times.The average recovery rate ranged from 75.5% to 84.7%.The relative standard deviations were between 2.0% and 10.5%.

关 键 词：丙草胺 高效液相色谱法 水质 土壤 水稻 

分 类 号：O657.72[理学—分析化学]