纳米壳聚糖纤维强化型磷酸钙骨水泥的研究  

A new strength-reinforced calcium phosphate cements

作　　者：许诺[1] 吴天一[1] 王国栋[1] 袁同洲[1] 黄野[1] 

机构地区：[1]南京医科大学第二附属医院,江苏南京210011

出　　处：《生物骨科材料与临床研究》2014年第1期58-60,67,共3页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：南京医科大学校基金项目(2010NJMU092)

摘　　要：目的探讨纳米壳聚糖纤维强化型磷酸钙骨水泥的机械及生物相容性能。方法使用纳米壳聚糖纤维强化CPC骨水泥,通过MTT比色法及DAPI染色观察其生物相容性情况;通过三点弯曲试验检测其力学性能。结果三点弯曲试验表明经过壳聚糖纤维强化后的骨水泥的机械性能(17.3±4.5)MPa较之普通骨水泥有明显改善(5.3±1.4)MPa;并且这样的改进对材料的生物相容性没有影响,其毒性试验显示新材料相容性比值为(97.5±3.3)%。细胞增值实验3天吸光值分别为(0.237±0.025)、(0.451±0.015)、(0.726±0.032)。结论新型纳米壳聚糖纤维强化型骨水泥无细胞毒性,具有良好的机械性能及生物相容性。Objective To evaluate themechanical behavior of the newcalciumphosphate bone cement（CPC）. MethodsThe CPCwas reinforced by chitosan fiber. The strength of the samples was investigated by a standard three-point bendingtest. Cell toxicity and proliferation of cells were also invested. Results The results of three-point bending test also showedthe incorporation of chitosan fiber（17.3±4.5）MPa significantly increased the mechanical strength of CPC（5.3±1.4）MPa. And the reinforcement is not harmful to the biocompatibility of CPC. Extracts of new bone cement showed noncytotoxic（97.5±3.3）%. The MTT activity of cells is （0.237±0.025）,（0.451±0.015）,（0.726±0.032） during 3 dayscultured. Conclusion CPC combined with chitosan fiber can meet the needs of strength and biocompatibility.

关 键 词：磷酸钙骨水泥 强化 壳聚糖纤维 

分 类 号：R318.08[医药卫生—生物医学工程]