烟碱对大鼠脑微循环中白细胞运动的影响  

Effect of Nicotine on Leukocyte Movement in Cerebral Microcirculation of Rat

作　　者：徐江平[1] 王传斌[2] 杨雪梅 芮耀诚[1] 

机构地区：[1]中国人民解放军第二军医大学药学院,邮政编码上海200433 [2]中国人民解放军第一军医大学南方医院肿瘤科

出　　处：《微循环学杂志》2000年第4期32-34,F004,共4页Chinese Journal of Microcirculation

基　　金：中国博士后科学基金部分资助!(中博基 [1998] 6号 )

摘　　要：目的 :观察烟碱对大鼠脑微循环中白细胞运动状态的影响。方法 :采用围堤式颅窗直视大鼠大脑软脑膜微循环 ,若丹明 6G标记循环血白细胞 ,微循环仪观察软脑膜微血管 (直径 2 5～ 40 μm ,长度 10 0μm)内白细胞的运动状态。结果 :烟碱能浓度依赖性的运动大鼠脑微血管内白细胞在内皮表面的滚动和粘附 ,预先皮下注射烟碱受体拮抗剂美加明可使滚动和粘附的白细胞分别降至 14 .2± 1.1个和 1.3± 0 .7个 (对照组为 10 .2± 1.2个 )。抗E 选择素单抗能明显阻断烟碱依赖所至的白细胞滚动 ,使滚动白细胞数目从 14 .2± 1.7个减为 2 .8± 0 .8个 ,而抗αvβ3整合素抗体能使粘附的白细胞数目从 10 .6± 1.0个降至 3 .8± 1.3个。结论 :烟碱可改变大鼠脑膜微循环中白细胞的运动状态 ,使白细胞在血管内皮表面滚动或粘附于其表面 ;烟碱的作用与激活脑烟碱受体有关 ;E 选择素介导烟碱引起的白细胞滚动 ,而αvβ3整合素则与烟碱引起的白细胞与血管内皮的粘附有关。Objective: Nicotine was examined for its ability to induce leukocyte rolling and adhesion in the cerebral microcirculation of rat.Method: A cranical window was surgically prepared for the visualzation for the pial microcirculation using an intra vital microscopy imaging system.Rhodamine labeled leukocytes could be visualized and video recorded as they traveled within the microvessels,and the quantitation of their rolling and adhesion along the pial venule walls was assessed during an off line video line video playback analysis.Results: Nicotine was found to produce significant concentration related increase in leukocyte rolling and adhesion mecamylamine,a antagonist,was found to inhibit the nicotine induced leukocyte rolling and adhesion.Anti E selectin monoclonal antibody blocked nicotine induced leukcyte rolling.While anti α vβ 3 antibody effectively inhibited leukocyte adhesion,but not rolling in similar experiments.Conclusion: These data suggest that nicotine,the principle pharmacological agent in cigarette smoke and related tobacco products acts via ganglionic type nicotinic receptor to enhance leukocyte rolling via E selectin leukocyte adherent via α vβ 3 integrin mechanisms in cerebral microcirculation of rat.

关 键 词：烟碱 脑微循环 白细胞人HUVEC 

分 类 号：R996[医药卫生—毒理学] R992[医药卫生—药学]