气味沙雷氏菌抑瘤活性组分研究  

Studies on the Antitumor Components of Serritia Odorifera

作　　者：彭武林[1] 王旭[2] 陈金国[1] 柴明胜[1] 

机构地区：[1]海军医学研究所,上海200433 [2]复旦大学微生物系

出　　处：《海军医学杂志》2000年第4期299-301,共3页Journal of Navy Medicine

摘　　要：目的 :研究气味沙雷氏菌粗核糖体制剂 (RP)中的抗肿瘤活性成分。方法 :以RP的多克隆抗血清做Western blot ,寻找制剂中特异性的免疫原 ;以DEAE 32离子交换层析法结合SephadexG 2 0 0层析法纯化之 ,体外细胞毒试验快速鉴定其抑瘤活性。结果 :RP及盐洗核糖体制剂 (SR)有两种特异性的免疫原 ,分子量分别为 16 .5kDa和 12kDa ,而层析核糖体制剂 (CR)中却没有 ;纯化的 16 .5kDa蛋白质印渍反应与RP中 16 .5kDa处的印渍反应一致 ;体外细胞毒性试验发现它对P388细胞的生长有抑制作用 ,IC50 可达 0 .0 0 1g/L ,但它不影响WISH细胞的生长。结论 :分子量为 16 .5kDa蛋白质是气味沙雷氏菌核糖体制剂体外抑瘤作用的有效成分。Objective: To explore the antitumor components of S. odorifera.Methods: Specific immunogen of S. odorifera were identified with multicloned antiserum of RP by Western blot, and were seperated and purified by DEAE ion changed chromatograph combined with Sephadex G 200 chromatograph. Results: Experiments proved that a 16.5 kDa protein and a 12 kDa protein are the specific immunogen of S. odorifera. The 16.5 kDa protein was seperated and purified, and this protein is cytotoxic to P 388 cell lines. Conclusion: The 16.5 kDa protein is an effective component of S. odorifera with antitumor activity in vitro.

关 键 词：气味沙雷氏菌 核糖体制剂 体外抑瘤作用 有效成分 

分 类 号：R979.1[医药卫生—药品]