HPLC同时测定防芷鼻咽颗粒中的丹皮酚和木兰脂素  

Simultaneous determination of paeonol and magnolin in Fangzhi nasopharyngeal granule by HPLC

作　　者：刘灵改[1] 支旭然[2,3] 任非[1] 郭进[1] 戎成才[1] 张兰桐[2] 

机构地区：[1]河北医科大学第二医院,河北石家庄050000 [2]河北医科大学药学院,河北石家庄050017 [3]河北省人民医院药学部,河北石家庄050051

出　　处：《华西药学杂志》2014年第3期311-313,共3页West China Journal of Pharmaceutical Sciences

基　　金：河北省科技支撑项目(编号:132777105D)

摘　　要：目的采用HPLC法同时测定防芷鼻咽颗粒中的丹皮酚、木兰脂素。方法采用Dikma Diamonsil C18色谱柱（250mm×4．6mm，5μm），柱温30℃，以甲醇-0．5‰甲酸水溶液为流动相，梯度洗脱，流速1mL·min^-1，进样量10μL；丹皮酚、木兰脂素的检测波长分别为274、278nm。结果丹皮酚8．203～265．5μg·mL^-1、木兰脂素7．938～254．0μg·mL^-1与峰面积的线性关系良好；平均加样回收率分别为98．59％、100．6％，RSD分别为1．43％、1．33％。结论所用方法简单、快速、可靠，可用于防芷鼻咽颗粒的质量控制。OBJECTIVE To develop a method for the simuhaneous determination of paeonol and magnolin in Fangzhi nasopharyngeal granule by HPLC. METHODS The samples were separated on a Diamonsil C18 column （250 mm × 4.6 mm, 5 μm） , by a gradient elution using methanol and 0.5‰ aqueous formic acid as the mobile phase at a flow rate of 1 mL·min^-1, the injection volume was 10μL. The column temperature was 30℃ , and the detection wavelengths of paeonol was 274 nm, magnolin was 278 nm. RESULTS The calibration curves of paeonol and magnolin showed good linearity at the ranges of 8. 203 -265.5,7. 938 -254.0 μg.mL^-1 and the average recoveries were 98.59% amd 100.6% with the RSD of 1.43% and 1.33% , respectively. CONCLUSION The method is simple, rapid, reliable and can be used for the drug control.

关 键 词：高效液相色谱法 防芷鼻咽颗粒 丹皮酚 木兰脂素 质量控制 

分 类 号：R917[医药卫生—药物分析学]