检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:李敏[1] 王晓梅[1] 热娜.卡斯木[1] 刘园园[1] 胡君萍[1]
机构地区:[1]新疆医科大学药学院,新疆乌鲁木齐830011
出 处:《华西药学杂志》2014年第3期314-316,共3页West China Journal of Pharmaceutical Sciences
基 金:国家科技支撑计划项目(2012BAI30B02)
摘 要:目的采用反相HPLC法同时测定中亚沙棘中的原儿茶酸、芦丁、异鼠李素。方法采用Gemini C18色谱柱(150mm×4.6mm,5μm),柱温25℃,流动相为甲醇-0.05%磷酸梯度洗脱(5:95→40:60→60:40),流速1.0mL·min^-1,检测波长256nm。结果中亚沙棘中原儿茶酸0.019~0,808μg(r=0.9998)、芦丁0.022~0.823μg(r=0.9998)、异鼠李素0.014—0.443μg(r=0.9999)与峰面积的线性关系良好;三者的平均加样回收率(n=9)分别为98.57%(RSD=1.57%)、99.13%(RSD=1.17%)、96.22%(RSD=1.81%)。结论所用方法简单、快速、高效,可为中亚沙棘的质量控制提供依据。OBJECTIVE To establish an RP - HPLC method for the simultaneous determination of protocatechuic acid, rutin and isorhamnetin in Hippophae rhamnoides L. subsp, turkestanica Rousi in. Ann.. METHODS The determination was carried out on a Gemini C18 column( 150 mm ×4.6 mm,5μm) with the column temperature at 25℃. The mobile phase was a mixture of methanol and 0.05% phosphoric acid water solution with the gradient elution(5 : 95→40 : 60→60 : 40). The flow rate was 1.0 mL·min^-1 and the detection wavelength was 256 nm. RESULTS The method showed a good linear relationship within the range of 0. 019 -0. 808μg for protocateehuic acid ( r = 0.9998 ) ,0. 022 - 0. 823 μg for rutin ( r = 0. 9998 ) and 0.014 - 0. 443μg for isorhamnetin ( r = 0. 9999 ). The average recoveries(n =9) were 98.57% with RSD of 1.57% ,99.13% with a RSD of 1.17% ,and 96.22 % with a RSD of 1.81% , respectively. CONCLUSION The method is simple, rapid and efficient, which provides the basis for quality control of H. rhamnoides.
关 键 词:中亚沙棘 原儿茶酸 芦丁 异鼠李素 含量测定 反相高效液相色谱
分 类 号:R917[医药卫生—药物分析学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.90