气相色谱法测定艳山姜中的桉油精  

Determination of cineol in Alpinia zerumbet by GC

作　　者：黄家宇[1] 李莉[1] 刘青[1] 盛钰[1] 张猛[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《华西药学杂志》2014年第3期326-327,共2页West China Journal of Pharmaceutical Sciences

基　　金：贵州省科技厅基金[黔科合(2010)3156]

摘　　要：目的采用气相色谱法测定艳山姜中桉油精的含量。方法用挥发油提取器回流提取，采用SE-54毛细管色谱柱（30m×0．53mm，0．5μm）分离，氢火焰离子化检测器检测，柱温为70℃，气化和检测温度为200℃，载气为氮气，流速为40mL·min^-1，进样量5μL，用外标法计算艳山姜药材中的桉油精。结果桉油精1．33～11．93μg与峰面积的线性关系良好（r=0．9998）；加样回收率为101．0％，RSD=1．45％（n=6）。结论所用方法准确、重复性好，可为艳山姜的质量标准研究提供参考。OBJECTIVE To develop a gas chromatography for determing of cineol in Alpinia zerumbet. METHODS Alpinia zerumbet was extracted with volatile oils extractor and separated with the capillary column SE - 54 （ 30 m × 0.53 mm, 0.5 μm） , and the content of cineol was detected by FID. The temperature was 70℃ ,injection chamber and detector temperature was 200 ℃, the carrier gas was N2 ,velocity of flow was 40 mL. min 1 , injection volume was 5μL. The conent of cineol was calculated by external standard method. RESULTS Cineole showed good linearity in the concentration range of 1.33 - 11.93 μg （ r = 0. 9998 ）, the average recovery rate was 101.0% with the RSD of 1.45%. CONCLUSION The method is accurate and reproducible. It can be used for the quality control of the cineol in Alpinia zerumbet.

关 键 词：艳山姜 桉油精 气相色谱 

分 类 号：R917[医药卫生—药物分析学]