香蕉GAPDH基因家族的生物信息学及转录表达分析  

Bioinformatics and Expression Analysis of Banana GAPDH Gene Family

作　　者：史后蕊 冯仁军[2] 王静毅[2] 柴娟[1,2] 任梦云 卢利方[2] 张银东[1] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2014年第6期1117-1123,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31201603);国家863计划项目子课题(No.2012AA101204-2);海南省自然科学基金项目(No.312050,No.312051);海南省研究生创新课题(No.Hys2012-18);海南省重大科技项目子课题(No.ZDZX2013023-1);中央级公益性科研院所基本科研业务费项目(No.ITBB130201)

摘　　要：从香蕉基因组数据库和NCBI数据库中检索香蕉GAPDH基因家族的所有成员,并采用生物信息学方法对其进行亚细胞定位、进化树分析和保守结构域预测。克隆测序发现,巴西蕉内的GAPDH基因家族成员序列与小果野蕉基因组数据库中提供的序列基本一致,同源性超过98%。转录表达分析表明,GAPDH基因家族成员在巴西蕉不同器官的表达模式多样:MaGAPCP1,MaGAPC6/8/10/11成组成型表达,其余的基因则在各组织器官间成差异型表达,且差异型表达的基因在不同组织器官中表达模式也不相同,组成型表达的基因在不同组织器官中表达模式虽相同,但表达量有差异。GAPDH基因家族成员在表达模式上的多样化,可能暗示其功能的多样化,这种多样化可能是在进化过程中为适应环境而出现的功能分化或冗余。结果为进一步研究GAPDH基因家族成员在香蕉生长、发育,非生物胁迫,果实后熟等重要生物学过程中的功能奠定基础。Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） is one of the key enzymes involved in glycolysis, gluconeogenesis and the Calvin cycle, which is widespread in higher plants playing an important role in plant growth and development. In this study, their subcellular location, phylogenetic tree and conserved domains prediction of the GAPDH gene family members from NCBI database and banana genome database were performed by the bioinformatics method. And all the members were amplified, cloned and sequenced from Musa AAA （Cavendish subgroup cv.Brazil）, showing 〉98% sequence identity with Musa acuminata genome. The results showed that the expression patterns of GAPDH gene family members were diversity in different tissues of Musa AAA （Cavendish subgroup or.Brazil）. MaGAPCP1 and MaGAPC6/8/lO/11 were constitutive expression genes, while the others were differentially expressed in different tissues. Although the expression patterns of constitutive expression genes were same, there were differences in expression levels. The diversity on the expression patterns of GAPDH gene family members may imply their functional diversity. Such diversification of biological function may be functions differentiation or redundant occurred in the evolution of GAPDH gene family. These results will lay the foundation for further research on the functions of GAPDH gene family in growth and development, abiotic stress, fruit ripening and other important biological processes of banana.

关 键 词：香蕉 GAPDH基因家族 生物信息学分析 表达分析 

分 类 号：S668.1[农业科学—果树学]