热研7-33-97袋装苗叶片愈伤组织诱导培养研究  

Callus Culture From Bagged Seedlings Leaves of Hevea Brasiliensis Müll. Arg. Clone Reyan 7-33-97

作　　者：欧阳超[1] 莫廷辉[1] 曾丽星[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]海南大学环植学院,海南海口570228

出　　处：《热带作物学报》2014年第6期1124-1130,共7页Chinese Journal of Tropical Crops

摘　　要：为扩大橡胶树组织培养外植体来源和建立高效稳定的橡胶树叶片离体再生体系,以1年生巴西橡胶树品种热研7-33-97袋装苗叶片为材料,研究叶片的预处理、0.1%升汞不同消毒时间、离体叶片切取部位、3种激素(6-BA、NAA、2,4-D)不同浓度配比对愈伤组织诱导的影响,筛选愈伤组织诱导最适培养基。结果表明:用液体培养基MS+3mg/L 6-BA+0.5 mg/L 2,4-D+0.2 mg/L NAA对橡胶树袋装苗叶片进行预处理,可诱导出愈伤组织;用0.1%升汞对袋装苗叶片消毒15 min,其存活率达2.47%,为最佳消毒方法;用含主脉叶块和含主脉出愈叶块未出愈伤组织部分进行培养所诱导的愈伤组织质地较坚实、呈黄绿色,为橡胶袋装苗叶片培养的最适宜外植体;橡胶袋装苗叶片最佳愈伤组织诱导培养基是MS+3 mg/L 6-BA+0.5 mg/L 2,4-D+0.2 mg/L NAA+4%蔗糖+3 g/L植物凝胶,用此培养基对含主脉叶块和含主脉出愈叶块未出愈伤组织部分进行培养,其出愈率分别达49.07%和42.22%。结论:大田1年生橡胶树品种热研7-33-97袋装苗叶片经预处理后选择含主脉叶块为外植体,可诱导出理想的愈伤组织。In order to expand the rubber tree explant sources and to establish efficient and stable rubber tree leaves in vitro regeneration system, the annual bagged seedling leaves of Reyan 7-33-97 were used as the explants to study on the effects of leaves pretreatment, different disinfection time with 0.1% HgC12, different parts of detached leaves, different combinations of three kinds of phytohormone （6-BA, NAA, 2,4-D） on callus induction frequency, and screen the best subculture medium. The results showed that leaves pretreated with liquid medium MS＋3 mg/L6-BA＋0.5 mg/L 2,4-D＋O.2mg/L NAA could induce callus; leaves disinfected with 0.1% HgC12 for 15 rain was the best disinfection method, its survival ratio could be 2.47%; leaves（with principal vein） and no callus part of leaves（with principal vein） already produced callus were the most suitable explants for rubber bagged seedlings leaves, callus induced by which showed tighten and yellow-green; the optimum callus induction medium was MS＋3 mg/L 6-BA＋0.5 mg/L 2,4-D＋0.2 mg/L NAA＋4% sucrose＋3 g/L phytagel, leaves （with principal vein） and no callus part of leaves （with principal vein） have already produced callus were cultured in this medium, their callus inducing ratio can amount to 49.07% and 42.22%. Conclusion： after pretreatment, choose leaves（with principal vein） of annual bagged seedling leaves of Reyan 7-33-97 as explants that can be used to induce ideal callus.

关 键 词：热研7-33-97 袋装苗 叶片 愈伤组织 

分 类 号：Q813.12[生物学—生物工程]