肾淋巴循环障碍促使单肾切除大鼠肾细胞凋亡  

Renal cell apoptosis induced by renal lymph circulation disorder in uninephrectomized rats

作　　者：张桃艳 蒋丽[2] 李德祥[3] 柳刚[4] 关广聚[4] 

机构地区：[1]济南护理职业学院临床学科,山东济南250000 [2]山东省淄博市中心医院预防保健科,山东淄博255000 [3]济南市第五人民医院普外科,山东济南250022 [4]山东大学第二医院肾内科,山东济南250033

出　　处：《中国现代医学杂志》2014年第12期1-6,共6页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30871163)

摘　　要：目的探讨肾淋巴循环障碍对大鼠肾脏细胞凋亡的作用,并初步探讨该作用与Bax、Bcl-2、Caspase3表达变化的关系。方法雄性Wistar大鼠48只,随机分为模型组和假手术对照组各24只。各组大鼠分别于术后第1、7、14及28天各处死6只,留取肾组织标本提取组织蛋白、mRNA和制作石蜡切片。运用Real-time PCR、Western Blot和免疫组化检测检测Bax、Bcl-2、Caspase3在肾脏组织的表达,用肾脏细胞原位凋亡(TUNEL)的方法检测肾脏凋亡细胞。结果细胞凋亡主要发生在扩张或萎缩的肾小管;模型组的凋亡指数在第14天达到高峰(P<0.01)。模型组的Bax和caspase-3蛋白和基因表达明显增强,主要位于小管间质,尤其在远端小管。相反,Bcl-2的表达明显减弱。Bax/Bcl-2比值明显增高。结论在肾淋巴循环障碍诱导的肾脏功能和结构改变的过程中,细胞凋亡显著增加,其中Bax/Bcl-2途径发挥了积极作用。【Objective】To investigate the influence of disorder of renallymph circulation on renal cell apoptosis and the expression of Bax/Bcl-2 and Caspase3 on rat kidney. 【Methods】Forty-eight male Wister rats were randomly divided into two groups, twenty-four rats in each group. Rats in each group were sacrifice at 7, l4, 28, 56 d after operation. Nephridial tissue were obtained for extracting protein, mRNA and paraffin section. Real-time PCR, immunostaining and western blot techniques were applied to detect the expression of Bax/Bcl-2, Caspase3 in renal tissue. Apoptotic nuclei were labeled using the terminal deoxynucleotidyl transferase dUTP nick-end-label（TUNEL） technique. 【Results】TUNEL exam showed that many positive staining cells in model group rats. Cell apoptosis mainly occurred in dilate and emarcid renal tubule. Apoptotic index elevated from day 1 in model group, peaked at day 14. Expressions of Bax and caspase-3 proteins and（or） mRNA were significantly increased in model group rats, mainly in renal tubulointerstitium. Oppositely, Bcl-2 was weakly. Increasing Bax to bcl-2 ratio were also detected in model group rats.【Conclusions】Renal lymph disorder enhanced renal cells apoptosis. Bax/Bcl-2 pathway mediates apoptosis in this disease.

关 键 词：淋巴管 肾脏 BAX BCL-2 CASPASE3 

分 类 号：R692.6[医药卫生—泌尿科学]