互叶白千层愈伤组织诱导及植株再生  

Induction of Callus and Regeneration in Melaleuca alternifolia

作　　者：王小敏[1] 莫昭展[1] 吴敏[1] 

机构地区：[1]玉林师范学院生命科学与生物技术学院,广西玉林537000

出　　处：《玉林师范学院学报》2014年第2期59-63,67,共6页Journal of Yulin Normal University

基　　金：广西科技攻关项目(桂科攻0992011-13)

摘　　要：本文报导了互叶白千层从无菌种子苗叶片诱导愈伤组织到植株再生的研究结果.实验表明:以继代代数不同的无菌苗叶片为诱导材料,在MS基本培养基中添加TDZ0.4mg/L+2,4-D0.4mg/L的第五代继代苗诱导效果较好,愈伤组织诱导率达100%.在互叶白千层愈伤组织分化阶段,在MS基本培养基中,激素组合筛选中以添加6-BA2.0mg/L+IAA2.5mg/L的分化效果较好,分化率高达94.53%.在分化苗伸长生根阶段,以MS+6-BA2.0mg/L+NAA0.8mg/L+AC 0.1%的效果较为显著,苗平均高度9.4cm,苗最壮,根粗且壮.In this study, the callus induction of aseptic seedlings and the plantlet regeneration of Melaleuca alternifolia were reported. The results showed that the optimization callus induction media was MS medium add TDZ0.4 mg/L＋2,4-D0.4mg/L used the fifth generation aseptic seedling and the induction rate achieved 100%. In the stage of callus differentiation, the best differential medium was MS medium supplied with 6-BA2.0mg/ L＋IAA2.Smg/L and differentiation rate of callus reached 94.53%. The best medium for regenerated plantlets rooting was MS ＋ 6-BA2.0mg/L＋NAA0.8mg/L ＋AC 0.1% and the average of seedling height reached 9.4cm. The seedlings and roots were strongest than those in the other medium.

关 键 词：互叶白千层 愈伤组织 诱导 分化 伸长与生根 

分 类 号：Q945.5[生物学—植物学]