2010-2012年广州市柯萨奇病毒A4、A10型VP1基因特征分析  

Sequence analysis of VP1 region of coxsackievirus A4 and coxsackievirus A10 in Guangzhou city, 2010-2012

作　　者：甄若楠 张颖[2] 谢华萍[2] 陈纯[3] 耿进妹[2] 和鹏[4] 狄飚[2] 王鸣[2] 

机构地区：[1]中山大学公共卫生学院,广州510080 [2]广州市疾病预防控制中心病毒免疫科 [3]广州市疾病预防控制中心传染病防治科 [4]广州市疾病预防控制中心微生物检验科

出　　处：《中华预防医学杂志》2014年第6期445-450,共6页Chinese Journal of Preventive Medicine

基　　金：国家科技重大专项（2012ZX10004213-005）;广州市医药卫生科技项目（20131A01110、20131A011117）

摘　　要：目的 检测广州市2010-2012年手足口病（HFMD）病例粪便样本中的肠道病毒,并对其中的柯萨奇病毒A4型（CoxA4）和柯萨奇病毒A10型（CoxA10） VP1基因核苷酸序列进行进化分析.方法 收集2010-2012年广州市HFMD疑似病例粪便样本5 484份,采用RT-PCR进行肠道病毒71型（EV71）、柯萨奇病毒A16型（CoxA16）、CoxA4、CoxA10和其他肠道病毒阳性检测,阳性样本共4 111份.对其中的CoxA4及CoxA10阳性样本分别进行巢式RT-PCR,扩增肠道病毒VP1基因片段,并对VP1基因片段进行测序,利用生物信息学分析序列,构建系统进化树,进行进化分析.结果 在4 111份肠道病毒阳性样本中,EV71、CoxA16、CoxA10、CoxA4的阳性率分别为35.1％（1443/4111）、30.7％（1261/4 111）、2.0％（82/4 111）、0.8％（31/4 111）,不同肠道病毒阳性率差异有统计学意义（x2=148.34,P＜0.05）.CoxA4样本以3岁组阳性率最高,为1.3％ （7/534）,CoxA10样本以0岁组阳性率最高,为3.7％（34/914）;CoxA4样本阳性率4月份达到高峰,为2.6％（12/460）,CoxA10样本阳性率8月份达到高峰,为4.3％（12/278）.VP1基因核苷酸遗传进化分析显示,CoxA4阳性样本VP1基因核苷酸序列可分为A、B2个亚型,Cox A10阳性样本VP1基因核苷酸序列可分为A、B、C3个亚型,广州市2010-2012年CoxA4和CoxA10阳性样本VP1基因核苷酸序列均属于A亚型.结论 广州市2010-2012年HFMD病例粪便样本中CoxA4和CoxA10阳性样本VP1基因核苷酸序列均属于A亚型.Objective To identify the enterovirus from stool samples of patients with haud, foot and mouth disease（HFMD） in Guangzhou from 2010 to 2012 and to perform phylogenetie analysis of the VP1 gene sequences of coxsackievirus A4 and coxsackievirus A10. Methods A total of 5 484 samples of suspected cases of HFMD which Guangzhou Center for Disease Control received from 2010 to 2012 were collected. Virus RNA was tested by nested RT-PCR method as human enterovirus 71, coxsackievirus A16, coxsaekievirus A4, coxsackievirus A10 and other enteroviruses positive, and 4 111 samples were positive. Phylogenetic tree was constructed by partial VP1 gene sequences of coxsackievirus A4 and coxsackievirus A10 to perform phylogenetic analysis. Results In 4 111 enterovirus-positive samples, the positive rate of EV71, CoxA16, CoxA10 and CoxA4 was 35.1% （ 1 443/4 111 ）, 30. 7% （ 1 261/4 111 ）, 2.0% （82/4 111 ） , 0. 8% （ 31/4 111 ） respectively. Different enterovirus-positive rate was statistically significant （X2 = 148. 34, P 〈 0. 05 ）. Incidences of coxsaekievirus A4 positive was highest in 3-year old children as 1.3% （ 7/534 ） , and that of coxsaekievirus AIO positive was highest in 0-year old children as 3.7% （34/914）. The highest positive rate of diagnosed coxsackievirus A4 positive cases was admitted in April（2. 6%, 12/460）, and the highest positive rate of diagnosed coxsackievirus A10 positive cases was admitted in August 4. 3% （ 12/278 ）. Phylogenetic analysis indicated that all the CoxA4 stains were divided into subtype A and subtype B, and the CoxA10 stains were divided into subtypes A, subtype B and subtype C. The VPI gene nucleotide sequences of CoxA4 and CoxA10 this study measured both belonged to subtype A. Conclusions The VP1 gene nucleotide sequences of CoxA4 and CoxA10 in Guangzhou from 2010 to 2012 both belonged to subtype A.

关 键 词：手足口病 逆转录聚合酶链反应 柯萨奇病毒感染 肠道病毒A型 人 

分 类 号：R725.1[医药卫生—儿科]