血管紧张素Ⅱ有效抑制大鼠高血压模型血管过氧化氢酶表达的实验研究  

The effects of Angiotention Ⅱ on Catalase expression of rat vessels

作　　者：沈凯[1] 袁国裕[1] 陈士良[1] 

机构地区：[1]浙江省舟山医院心内科,316000

出　　处：《心电与循环》2014年第3期233-234,257,I0001,共4页Journal of Electrocardiology and Circulation

基　　金：浙江省医药卫生科技计划(项目编号:2011KYA170)

摘　　要：目的探讨血管紧张素Ⅱ(Angll)对大鼠血管过氧化氢酶(CAT)表达的影响。方法利用Angll微泵灌注制备高血压大鼠模型,分为Anll组、生理盐水组、对照组,每组6只。分别检测0、3、7、14d后各组大鼠尾动脉收缩压;连续灌注14d后,取材观察各组大鼠血管形态学改变;并采用免疫组化法检测各组大鼠颈动脉血管中CAT及氧化应激产物4-羟烯酸(4HNE)蛋白的表达情况。结果与生理盐水组及对照组相比,Angll灌注后能够显著提高大鼠尾动脉收缩压水平及颈动脉血管中膜厚度(均P<0.01);免疫组化显示Angll组颈动脉血管CAT表达显著降低,而4HNE表达显著增加(均P<0.01)。结论 AngⅡ可下调大鼠血管中CAT表达而导致氧化应激的发生。Objective To investigate the effects of Angiotensin （Ang）Ⅱon the expression of catalase of rat vessels. Methods 18 rats were randomly divided into Ang Ⅱ group （n=6）infused with Ang Ⅱ by micro- pump and saline group （n=6） infused with saline for 14 days, and control group（n=6）. Systolic pressure of tail artery was measured at 0,3,7 and 14 day. The rats were sacrificed for observing vessel morphological changes at 14th day. Catalase and oxidative stress product- 4HNE were determined by immunohistochemistry. Results Systolic blood pressure of tail artery as wel as the thickness of carotid artery media increased significantly, Catalase was significantly lower while oxidative stress product- 4HNE was higher in Ang Ⅱ group compared to control group （al P〈0.01）. Conclusion AngⅡ may promote oxidative stress through inhibiting catalase expression of rat vessels.

关 键 词：高血压 血管紧张素Ⅱ 过氧化氢酶 

分 类 号：R544.1[医药卫生—心血管疾病]