新疆石河子奶牛隐性乳房炎源金黄色葡萄球菌的分离鉴定及药物敏感性分析  

Isolation,Identification & Drug Sensitivity Test of Staphylococcus Aureus of Cow Recessive Mastitis in Shihezi Area,Xinjiang

作　　者：欧都[1] 齐向涛[1] 许追[1] 许丹丹[1] 杨莉[1] 齐亚银[1] 

机构地区：[1]石河子大学动物科技学院,石河子832000

出　　处：《中国奶牛》2014年第10期29-32,共4页China Dairy Cattle

基　　金：石河子大学高层次人才科研启动专项(RCZX201106);国家自然基金(36260610)

摘　　要：为了解石河子地区部分规模化奶牛场奶牛隐性乳房炎源金黄色葡萄球菌的生物学特性及药物敏感性,为科学防治该病的发生提供参考依据,本试验选择了石河子周边地区不同规模的11个奶牛场,通过采用LMT法检测其隐性乳房炎的感染率,并从采集到的313份隐性乳房炎奶样中分离鉴定金黄色葡萄球菌,同时采用纸片扩散法(K-B法)对分离株的药物敏感性进行了分析。结果表明,试验区奶牛隐性乳房炎的总阳性率为44.56%(869/1950),金黄色葡萄球菌的分离率为44.71%(93/208);药敏试验结果表明,大部分菌株对阿莫西林、红霉素、四环素表现为高度耐药,耐药率分别为92.30%(24/26)、80.77%(21/26)、80.77%(21/26);对庆大霉素、氧氟沙星、头孢唑啉表现为敏感,敏感率分别为57.70%(15/26)、76.92%(20/26)、76.92%(20/26);部分菌株对克林霉素中度敏感,敏感率为46.15%(12/26)。建议在选用抗生素治疗奶牛隐性乳房炎时,以流行菌株的药物敏感性为依据。The aim of this study was to understand biological characteristics and drug resistance of staphylococcus aureus in cow recessive mastitis from large-scale dairy farms in Shihezi region in order to provide reference to scientific prevention. Using LMT method, this experiment chose 11 large-scale dairy farms in Shihezi region to test the infection ratio of recessive mastitis and staphylococcus aureus from 313 recessive mastitis milk samples were separated and identified. At the same time, this experiment used the disc diffusion method （K-B method） to analyze the drug resistance of isolated bacterial strains. Research results showed that the total positive rate of cow recessive mastitis in experimental dairy farms was 44.56% （869/1950）, the separation rate of staphylococcus aureus was 44.71% （93/208）. Drug susceptibility test results showed that the majority of strains were highly resistant to amoxicillin, erythromycin, tetracycline, and drug resistance rate was respectively 92.30%（24/26）, 80.77%（21/26）, 80.77%（21/26）. The samples showed sensitive to gentamicin, ofloxacin, cefazolin and the susceptibility rate was respectively 57.70%（15/26）,76.92%（20/26）, 76.92%（20/26）. Some strains were moderately sensitive to clindamycin and the susceptibility rate was 34.62%（6/26）. Thus it is suggested advice should be given according to drug sensitivity of epidemic strains when choosing antibiotics to treat cow recessive mastitis.

关 键 词：隐性乳房炎 金黄色葡萄球菌 药敏试验 

分 类 号：S858.23[农业科学—临床兽医学]