Ag(Ⅲ)流动注射化学发光体系测定磺胺甲噁唑  

Determination of sulfamethoxazole in human urine and serum by flow injection-chemiluminescent system based on Ag(Ⅲ) complex

作　　者：石红梅[1] 王莎莎[1] 代婷婷[1] 林华萍[1] 崔晶晶[1] 康维钧[1] 

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017

出　　处：《中国卫生检验杂志》2014年第10期1379-1381,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(81273128;81172722)

摘　　要：目的建立磺胺甲噁唑化学发光检测新方法。方法碱性介质中磺胺甲噁唑抑制Ag(III)配合物-鲁米诺发光体系的发光强度,且抑制强度与样品浓度在一定范围内呈良好的线性关系,由此建立Ag(Ⅲ)流动注射化学发光体系测定磺胺甲噁唑新方法。结果优化条件下,方法检出限(3σ)为3.0×10-10mol/L,在3.0×10-8mol/L^9.0×10-7mol/L和3.0×10-5mol/L^9.0×10-4mol/L范围内成良好线性关系。取3.0×10-5mol/L磺胺甲噁唑进行11次平行测定,相对标准偏差(RSD)为1.08%。结论该方法简便、准确,可用于尿液及血清中磺胺甲噁唑的测定。Objective To establish a new method for determination of sulfamethoxazole by flow injection （FI） - chemilumines- cent（CL） system. Methods The CL intensity of Ag（Ⅲ） -Luminol system could be significantly inhibited by sulfamethox- azole under alkaline conditions, and the strength of inhibition showed good linearity with the concentrations of sulfamethoxazole within a certain range. Based on this, a new method of FI - CL system for detection of sulfamethoxazole was developed. Results Under the optimized conditions, the linear range of the method were 3.0 ×10^-8 mol/L - 9.0×10^-7 mol/L and 3.0 ×10^-5 mol/L - 9.0 ×10^-4 mol/L with a detection limit of 3.0 x 10 -10 mol/L （3σ）. The RSD （ n = 11 ） was 1.08% for 3.0 ×10^-5 mol/L of sulfamethoxazole. Conclusion The method is simple, accurate and it can be used for determination of sulfamethox- azole in urine and serum.

关 键 词：磺胺甲噁唑 Ag(Ⅲ)配合物 化学发光 抑制 测定 

分 类 号：O657.3[理学—分析化学]