DHBV DNA在鸭胰脾组织中原位杂交的研究  

Experimental study on DHBV DNA in duck's pancreas and spleen tissues by in situ hybridization

作　　者：彭凤英[1] 郭树华[2] 

机构地区：[1]重庆医科大学临床学院传染科,重庆400016 [2]重庆医科大学第二临床学院传染科,重庆400010

出　　处：《重庆医科大学学报》2001年第1期60-62,共3页Journal of Chongqing Medical University

摘　　要：目的：对慢性DHBV感染鸭的肝、胰、脾组织 DHBV DNA作对比研究。方法：采用原位杂交技术。结果：DHBV不仅可以感染鸭肝细胞，且可感染胰、脾组织，DHBV DNA 阳性细胞的分布密度以肝组织最高，胰脾次之。结论：DHBV具有组织器官泛嗜性，但其在肝外组织中的复制似不及在肝细胞中活跃。Objective: To study the difference of DHBV DNA in the duck's liver, pancreas and spleen tissue. Methods : In situ hybridization using digoxigenin-labeled DHBV DNA C gene probe. Results: DHBV DNA was present not only in hepatocytes but also in the pancreatic acini , in cells of splenic red pulp and in the splenic germinal center. The DHBV DNA positive cells appeared more frequently in liver than in spleen and pancreas. Conclusion: It suggests that DHBV is also tropic for extrahepatic tissue, but it may replicate more actively in liver than in the extrahepatic tissue.

关 键 词：鸭乙型肝炎病毒 原位杂交 DNA探针 地高辛标记 DHBV-DNA 

分 类 号：R512.62[医药卫生—内科学] R858.322.6[医药卫生—临床医学]