肉苁蓉多糖的提取工艺优化  

Optimization on Extraction Technology of Desertliving Cistanche Polysaccharides

作　　者：肖道安[1] 

机构地区：[1]宜春学院化学与生物工程学院,宜春336000

出　　处：《世界科学技术-中医药现代化》2014年第4期926-929,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江西省科学技术厅重点科技计划项目(赣财教字[2007]173号):高纯度乌索酸的提取工艺研究;负责人:李开泉

摘　　要：目的:研究肉苁蓉多糖的提取工艺,为肉苁蓉的开发利用提供依据。方法:采用蒽酮-硫酸法测定肉苁蓉多糖含量,以肉苁蓉多糖得率为指标,比较不同的提取方法,通过正交试验优化提取工艺。结果:采用吐温60协同超声提取肉苁蓉多糖效果较好,其最佳提取工艺:表面活性剂为0.5%吐温60,料液比1∶25,温度60℃,提取时间30 min。肉苁蓉多糖的得率为8.17%。结论:该提取工艺合理可靠,有利于肉苁蓉的开发利用。By studying on extraction technology of desertliving cistanche polysaccharides, it provided evidences for development and utilization of cistanche. Anthrone-sulfuric acid method was used in the measurement of desertliving cistanche polysaccharides. The yield of desertliving cistanche polysaccharides was used as index. Different types of extraction methods were compared. Then, the extraction process was optimized by orthogonal experiment. The results showed that the best extraction process was Tween-60 ultrasonic cooperated. The best extraction process was when the mass concentration of Tween-60 was 0.5%, solid-to-liquid ratio was 1：25, temperature was 60℃, and the extraction time was 30 min. The extraction yield of desertliving cistanche polysaccharides was 8.17%. It was concluded that this extraction technology was reasonable and reliable, which can be used in the development and utilization of cistanche.

关 键 词：肉苁蓉 多糖 提取工艺 蒽酮硫酸法 

分 类 号：R284.2[医药卫生—中药学]