基于碳纳米角-阿霉素的新型载药体系的制备和体外释放行为  

Preparation and in vitro release behavior of a new drug delivery system based on single-walled carbon nanohorns-doxorubicin

作　　者：钟文英[1] 马小娜[1] 舒畅[1] 庞莉莉[1] 郭劲[1] 

机构地区：[1]中国药科大学理学院分析化学教研室药品质量与安全预警教育部重点实验室,南京210009

出　　处：《中南药学》2014年第5期402-406,共5页Central South Pharmacy

基　　金：国家自然科学基金(No.81183023)

摘　　要：目的制备一种以壳聚糖(CS)为修饰剂氧化单壁碳纳米角(oxSWCNHs)介导的载阿霉素(DOX)的新型药物转运系统(DOX@oxSWCNHs/CS),并考察理化性质及体外释放行为。方法制备DOX@oxSWCNHs/CS载药体系,考察体系在水、PBS、细胞培养基中的分散性,使用热重分析(TGA)、透射电镜(TEM)、紫外可见吸收光谱、荧光光谱、zeta电位对其理化性质进行考察,评价其体外释放效果。结果 DOX通过π-π堆积作用装载于oxSWCNHs上,载药量达60%;用CS修饰oxSWCNHs的疏水表面,可增加oxSWCNHs在水溶液中的分散性,特别是在盐溶液中分散性。载药体系的体外释放具有pH依赖性和缓释效果。结论 oxSWCNHs能够作为一种潜在的阿霉素载体达到药物的缓释效果。Objective To prepare and characterize a new oxidized single-wall carbon nanohorns （oxSWCNHs） -mediated drug delivery system （DOX@oxSWCNHs/CS） which loads doxorubicin （DOX） and is modified by chitosan （CS）, and evaluate their in vitro release quality. Methods DOX@oxSWCNHs/CS nanocarrier was prepared and the dispersity in water, PBS, and cell culture medium was estimated. The characteristics of DOX@oxSWCNHs/CS were evaluated by the thermogravimetry analysis （TGA）, transmission electron microscopy （TEM）, UV-vis absorbance spectra, fluorescence spectra and zeta potential. Drug release behaviors were also investigated. Results DOX was attached to oxSWCNHs surface via π-π stacking interaction, the weight percent of DOX in DOX@oxSWCNHs was 60%, followed by encapsulation of oxSWCNHs with CS to enhance the stability of nanocarrier in the aqueous medium, especially in the salt solution. The release of drug on nanocarrier was pH-dependent and controlled. Conclusion oxSWCNHs can act as a potential DOX carrier for sustained drug release.

关 键 词：单壁碳纳米角 阿霉素 壳聚糖 分散性 体外释放 

分 类 号：R943[医药卫生—药剂学]