高效液相色谱法测定肉豆蔻八味胶囊中去氢二异丁香酚的含量  

Determination of dehydrodiisoeugenol in Roudoukou Bawei capsules by HPLC

作　　者：王文静[1] 张薇薇[1] 王玉华[1] 

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110

出　　处：《中南药学》2014年第5期470-472,共3页Central South Pharmacy

基　　金：内蒙古自治区科技创新引导奖励资金项目(No.20121502)

摘　　要：目的 建立肉豆蔻八味胶囊中去氢二异丁香酚的含量测定方法。方法 色谱柱为Phenomen Kromasil C18（4.6 mm×250 mm,5μm）;流动相为甲醇-水（67：33）;检测波长为274 nm。结果 去氢二异丁香酚在70.40～704.0 ng与峰面积积分值呈良好的线性关系,回归方程为y=1.366×104x-2.513×104（r=0.999 9）,低、中、高浓度平均加样回收率为97.2%、98.1%、99.1%,RSD为0.82%、0.48%、0.66%（n=3）。结论 该方法简便,结果准确,可用于肉豆蔻八味胶囊中去氢二异丁香酚的含量测定。Objective To establish an HPLC method to determine the content of dehydrodiisoeugenol in Roudoukou Bawei capsules. Methods Phenomen Kromasil C18 column （4.6 mm×250 mm, 5 μm） was used; the mobile phase was methanol-water （67 ： 33）; and the detective wavelength was 274 nm. Results The calibration curve was linear at 70.40 - 704.0 ng, y = 1.366 × 10^4x - 2.513 × 10^4 （r = 0.999 9）. The average recoveries were 97.2%, 98.1% and 99.1%, and RSDs were 0.82%, 0.48% and 0.66% （n = 3）. Conclusion This method is simple, accurate and suitable for the quality control of Roudoukou Bawei capsules.

关 键 词：肉豆蔻八味胶囊 去氢二异丁香酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]