血管活性物质对人内皮细胞C-型利钠利尿肽合成和释放的影响  

The effect of vasoactive substances on synthesis and release of C-type natriuretic peptide in cultured human endothelial cells

作　　者：王晓红[1] 陈亚红[1] 齐永芬[1] 李淑莲[1] 庞永政[1] 刘乃奎[1] 唐朝枢[1] 

机构地区：[1]北京医科大学第一医院心血管研究所,北京100034

出　　处：《中国病理生理杂志》2001年第3期193-195,共3页Chinese Journal of Pathophysiology

基　　金：九五国家科技攻关项目 (No.96 - 90 6 - 0 2 - 0 7)

摘　　要：目的 :在培养的人内皮细胞上观察内皮素 (ET)、血管紧张素Ⅱ (AngⅡ )和同型半胱氨酸 (Hcy)对C -型利钠利尿肽 (CNP)生成和释放的影响 ,以探讨CNP生成和释放的机制。方法 :人内皮细胞培养 ;CNP放免测定。结果 :ET、AngⅡ均可刺激内皮细胞CNP的生成 ,10 -9、10 -8、10 -7mol/L的ET和AngⅡ分别使细胞CNP含量较对照组高 1% (P >0 .0 5 ) ,49% (P <0 .0 5 ) ,117% (P <0 .0 1)和 137% (P <0 .0 1) ,16 5 % (P <0 .0 1) ,2 0 1% (P <0 .0 1) ,大剂量ET、AngⅡ可刺激CNP的释放。Hcy对内皮细胞CNP的生成没有影响 ,但 10 -9、10 -8、10 -7mol/LHcy可使其释放增加 17% (P >0 .0 5 ) ,84% (P <0 .0 1)和 5 5 5 % (P <0 .0 1)。结论 :ET、Ang和Hcy可调节CNP的释放和AIM: To investigate the effect of endothelin (ET), angiotensin II (AngII) and homocysteine (Hcy) on C-type natriuretic peptide (CNP) synthesis and release. METHODS: Human endothelial cell was cultured; CNP was measured by radioimmunoassay method. RESULTS: ET and AngII could augment CNP synthesis in human endothelial cells. Compared with control group, 10 -9 ,10 -8 ,10 -7 mol/L ET and Ang II increased CNP content of endothelial cells by 1%( P >0.05), 49%( P <0.05),117%( P <0.01) and 137% ( P <0.01),165%( P <0.01),201%( P <0.01),respectively. A great dose of ET and Ang II also stimulated CNP release from cultured human endothelial cells. Hcy had no effect on CNP synthesis, but 10 -9 ,10 -8 ,10 -7 mol/L Hcy enhanced CNP release from cultured human endothelial cells by 17%( P >0.05),84%( P <0.01) and 555%( P <0.01), respectively. CONCLUSION: ET, AngII and Hcy might be involved in the synthesis and release of human endothelial cell CNP.

关 键 词：利尿钠激素 内皮缩血管肽类 血管紧张素类 高半胱氨酸 CNP ET ANG Hcy 血管内皮细胞 

分 类 号：R543.02[医药卫生—心血管疾病]