国产蛇毒激活血浆蛋白C的研究  

Identification of protein c activator from nine species of Chinese snake venoms

作　　者：孙林光[1] 管锦霞[1] 黄劭[1] 余清声[1] 

机构地区：[1]广州医学院广州蛇毒研究所,广东广州510182

出　　处：《中国病理生理杂志》2001年第3期241-244,共4页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目;广州市教委资助项目

摘　　要：目的 :从 9种国产蛇毒中筛选具有激活血浆蛋白C作用的蛇毒。方法 :运用活化部分凝血活酶时间(APTT)和发色底物实验分别观察抗凝活性和酰胺酶活性 ,综合抗凝活性和酰胺酶活性确定蛋白C蛇毒激活作用。结果 :在 9种国产蛇毒中烙铁头蛇毒及蝮蛇毒在 1 5mg/L浓度下即使人血浆纯蛋白C产生酰胺酶活性 ,并使APTT显著延长。结论 :9种国产蛇毒中烙铁头蛇毒及蝮蛇毒具有激活人体血浆蛋白C成为活化蛋白C(APC)AIM:To determine which species of snake venoms contained protein c activator among 9 species of Chinese snake venoms. METHODS: Anticoagulant activity was examined by activated partial thromboplastin time (APTT) assay,and amidolytic activity was measured with activated protein c (APC) specific chromogenic peptide substrate-chromozy APC. RESULTS: Among 9 species of Chinese snake venoms,Trimeresurus mucrosquamatus venom and Agkistrodon halys venom were not only able to generate amidolytic activity from purified human PC, but also prolonged APTT strongly even at such a concentration as 1.5 mg/L.CONCLUSION: Trimeresurus mucrosquamatus venom and Agkistrodon halys venom contain protein c activator which activating human plasma PC into APC.

关 键 词：蛇毒液类 血浆 蛋白质C 酶激活 

分 类 号：R996.3[医药卫生—毒理学]