进境韩国大花蕙兰上唐菖蒲伯克氏菌的分离鉴定及生物学研究  

Isolation,identification and biological characterization of Burkholderia gladioli in Cymbidium imported from Korea

作　　者：厉艳[1] 甘琴华[1] 封立平[1] 邵秀岭 

机构地区：[1]山东出入境检验检疫局检验检疫技术中心,青岛266002

出　　处：《植物病理学报》2014年第3期313-317,共5页Acta Phytopathologica Sinica

基　　金：国家质检总局项目(2010IK270)

摘　　要：大花蕙兰（Cymbidium hybridum），又名虎头兰、喜姆比兰，兰科、兰属多年生草本植物，是近几年我国花卉市场上流行的高档室内盆栽花卉。大花蕙兰的杂交育种已有一百多年的历史，每年新增加的品种有几十种之多。近年来，我国检验检疫部门分别从各入境口岸的进境大花蕙兰中多次发现携带有菊基腐病菌（Erwinia chrysanthemi）、兰花细菌性褐腐病菌（Erwinia cypripedii）、建兰花叶病毒（Cymbidium mosaic virus）等多种病原生物，均有潜伏期长、发病率高等特点。入境大花蕙兰种苗携带植物病原细菌、病毒等有害生物隐蔽性强、风险较高。Three pathogenic bacterial strains were isolated from leaves of Cymbidium imported from Korea using flat-panel method. The strains were identified and their biological characteristics were studied. Studies on morphological, cultural, physiological and biochemical characteristics showed that all strains formed white, glossy, convex, opaque and round single colony on nutrient agar （NA） and secreted a significant amount of yellowish green, non-fluorescent diffusible pigment. All of these bacteria are Gram-negative. Bacterial cells are straight or slightly curved rod-shaped, with 1 to 2 polar flagella under the microscope. According to Biolog, 16S rDNA gene sequences analysis, PCR and pathogenicity test, the three strains were identified as Burkholderia gladiol. This is the first report of B.gladiol infecting Cymbidium.

关 键 词：大花蕙兰 分离鉴定 进境 生物学 唐菖蒲 建兰花叶病毒 多年生草本植物 韩国 

分 类 号：S682.31[农业科学—观赏园艺]