海南甘蔗病毒病的调查及其病原分子鉴定  

Survey and molecular detection of sugarcane virus diseases in Hainan Province

作　　者：王洪星[1,2] 张雨良[1] 余乃通[1] 罗志文[3] 王建华[1] 杨文君[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]海南大学应用科技学院(儋州校区),儋州571737 [3]海南省农业科学院热带果树研究所,海口571101

出　　处：《植物病理学报》2014年第3期323-326,共4页Acta Phytopathologica Sinica

基　　金：海南省重大科技专项课题(ZDZX2013023-1-23);中央级公益性科研院所基本科研业务费专项资金课题(ITBB110303);国家现代农业产业技术体系建设专项资金(nycytx-24)

摘　　要：海南是我国重要的甘蔗生产省份之一，但其甘蔗主要种植区感染病毒的种类和数量尚不十分清楚，且海南甘蔗花叶病病原病毒缺乏分子水平的系统鉴定。为明确海南甘蔗病毒病的种类、数量、分布及危害情况，本研究拟建立较为完整的甘蔗病毒病检测技术体系，对海南甘蔗病毒病展开调查，为甘蔗抗病毒基因工程及健康种苗发展提供参考。In order to identify the viruses that infected sugarcane in Hainan, fourteen pairs of primer specific to SCMV, SrMV, MDMV, JGMV, SCBV, SSV, SCSV, SDV, SFDV, ScYLV and ScSMV were designed and used to detect 108 sugarcane samples which might be infected by virus diseases（including 20 experimental varieties）, Ten health samples from 8 main cultivars in Hainan island were used as negative controls. RT-PCR products showed that 42 samples were SrMV infection with the rate of 38.8 %, 24 samples of ScYLV infection with the rate of 22.2% and SrMV and ScYLV co-infection with the rate of 6.4%. The result indicates that SrMV is the major pathogen of sugarcane mosaic disease in Hainan Province. The study provides a scientific basis for sugarcane virus detection and virus-free seedling cultivation.

关 键 词：甘蔗生产 分子鉴定 病原病毒 病毒病 海南 抗病毒基因工程 甘蔗花叶病 分子水平 

分 类 号：S566.1[农业科学—作物学]