西芹腐根浸提液处理后黄瓜枯萎病菌致弱机制  

Attenuation Mechanism of Parsley Rotten Root Extract on Fusarium oxysporum f. sp. cucumerinum

作　　者：李杰[1] 云兴福[1] 高晓敏[1] 王勇[1] 钱程[1] 马立国[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《科技导报》2014年第14期24-30,共7页Science & Technology Review

基　　金：国家自然科学基金项目(31160100);内蒙古自治区应用技术研究与开发项目(20110711)

摘　　要：室内条件下用西芹腐根乙醇、丙酮和蒸馏水浸提液,连续5代处理黄瓜枯萎病菌,测定处理后黄瓜枯萎病菌分泌的镰刀菌酸量和细胞壁降解酶活性及其与致病力的相关关系,以揭示西芹腐根浸提液连续多代处理黄瓜枯萎病菌的致弱机制。结果表明,各处理的致病力随接种代数的增加逐渐减小,镰刀菌酸质量浓度和果胶酶活性随培养代数的增加逐代减少,纤维素酶活性随培养代数的增加无明显的变化规律,β-葡萄糖苷酶活性随培养代数的增加逐代升高,但均表现为处理低于对照。相关分析表明,致病力与菌体分泌的镰刀菌酸质量浓度和果胶酶活性均表现为显著或极显著的正相关关系,与纤维素酶活性相关性不显著;与培养第3~5代β-葡萄糖苷酶活性表现为显著或极显著的正相关关系。This paper investigates the attenuation mechanism of parsley rotten root extract on Fusarium oxysporum f. sp. cucumerinum（Foc.）. Foc. was treated from the first to fifth generation with parsley rotten root extract prepared by ethanol, acetone, and distilled water. The excretive fusarium acid content and cell wall degradation enzyme activity were detected and their correlation with pathogenicity was studied. The results show that the pathogen＇s fusarium acid content and pectinase enzyme activity were significantly reduced with the increase of treated generations, the cellulose enzyme activity had no apparent change with the increase of treated generations, and the β-glycosidase enzyme activity increased with the increase of treated generations, but all were lower than those in the control group. Correlation analysis shows that there are significant or extremely significant positive correlation between pathogenicity and the pathogen＇s fusarium acid content, pectinase enzyme activity, and β-glycosidase enzyme activity, but there is no clear relationship between pathogenicity and the cellulose enzyme activity.

关 键 词：西芹 腐根浸提液 镰刀菌酸 细胞壁降解酶 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治]