生物阻抗频谱技术检测Wistar大鼠体液成分和脂肪分布的初步研究  

Preliminary Study of Body Fluid Composition and Fat Distribution by Bio-impedance Spectroscopy( BIS) in Wistar Rats

作　　者：董晓敏[1] 李嘉辉[2] 张伟娇 田雪子 芦玲巧[1] 童学红[1] 郝刚[1] 

机构地区：[1]首都医科大学基础医学实验教学中心,北京100069 [2]首都医科大学

出　　处：《实验动物科学》2014年第2期25-28,共4页Laboratory Animal Science

基　　金：首都医科大学校长基金;(No:13JYY13)

摘　　要：目的探讨应用生物阻抗分析仪测量不同性别Wistar大鼠体液成分和脂肪分布情况。方法利用生物阻抗分析仪检测Wistar大鼠身体组份,包括体液总量(TBW)、细胞内液(ICF)、细胞外液(ECF)、脂肪量(FM)、除脂肪量(FFM)、体液总量/体质量(TBW/BW)、细胞外液量/体质量(ECF/BW)、细胞内液量/体质量(ICF/BW)、脂肪量/体质量(FM/BW)和除脂肪量/体质量(FFM/BW)。雄性组13只,雌性组13只分别检测各项指标改变,以证明不同性别正常成年Wistar大鼠身体组份构成。结果不同性别两组间比较,雄性Wistar大鼠体液总量、细胞内外液含量、TBW/BW、ECF/BW、ICF/BW高于雌性Wistar大鼠,P值均小于0.05,差异有显著性。FM/BW、FFM/BW比较,P值均小于0.05,两组差异有显著性。雌性Wistar大鼠的脂肪含量高于雄性Wistar大鼠。结论正常雄性成年Wistar大鼠的体液含量高于雌性Wistar大鼠,而雌性Wistar大鼠脂肪百分比高于雄性。Objective To study the measurement of body fluids composition and fat distribution in different gender Wistar rats by Applying BioImp Vet Body Composition. Method Body fluids components of Wistar rats including total body fluid （TBW） , intracellular fluid （ICF）, extracellular fluid （ECF）, fat mass （FM） , fat-free mass （FFM） ,TBW/BW, ECF/BW, ICF/BW, FM/BW, FFM/BW. According to gender, normal adult Wistar rats were divided into two groups, male group （n = 13）, female group （n = 13） to detect. Result There were significant differences between the male Wistar rats group and the female group. The body fluids of male Wistar rats were higher than the female. Compared FM / BW, FFM / BW of the two groups, the difference was significant （P 〈 0.05）. The percentage of fat content in the female Wistar rats was higher than the male. Conclusion The body fluids composition of the normal adult male Wistar rats was higher than the female. The percentage of fat content in the female Wistar rats was higher than the male.

关 键 词：生物阻抗 WISTAR大鼠 体液成分 脂肪分布 

分 类 号：Q95-33[生物学—动物学]