云南杓兰和紫点杓兰菌根真菌rDNA ITS序列及共生专一性分析  

Mycorrhizal Fungus rDNA ITS and Specificity of Cypripedium yunnanens and Cypripedium guttatum

作　　者：赵欣宇[1] 缪福俊[2,3,4] 李璐[1] 王娟[2,3,4] 

机构地区：[1]西南林业大学,云南昆明650224 [2]云南省林业科学院,云南昆明650201 [3]国家林业局重点开放性实验室云南珍稀濒特森林植物保护和繁育实验室 [4]云南省森林植物培育与开发利用重点实验室,云南昆明650201

出　　处：《西部林业科学》2014年第3期57-61,共5页Journal of West China Forestry Science

基　　金：国家林业公益性行业科研专项项目(201204110);云南省社会事业发展专项(2010CA010):云南藏区典型区域生态安全与防控技术研究及应用示范;云南省应用基础研究重点项目(2013FA054);云南省中青年学术技术带头人后备人才培养项目(2010CI016);云南省教委湿地生态学创新团队项目;云南省应用基础研究计划青年项目(201401YC00439)等资助

摘　　要：杓兰属植物具有很高的观赏和药用价值,但因被过度采集,已成为濒危植物。菌根真菌是杓兰栽培与繁育是否成功的关键因子。本实验采用rDNA ITS序列扩增技术和MEGA软件对滇西北的云南杓兰和紫点杓兰的菌根真菌进行研究,结果表明:云南杓兰和紫点杓兰的菌根真菌存在显著的物种层面的专一性关系,即菌根真菌与杓兰属植物有较强的共生趋势。分离得到的菌根真菌序列已上传至NCBI,登录号为lcl51879、lcl25153、lcl48033、lcl38377、lcl14203、lcl24557、lcl52287以及lcl46937。Wild lady’s slipper orchids （Cypripedium calceolus） as an ornamental plant with highly medicinal value, are being seriously threatened due to over collection , and are becoming endangered species .For a success cultivation and breeding of Cypripedium, mycorrhizal fungus is playing a very important role in this process .In this test , mycorrhizal fungus extracted from C.yunnanense and C.guttatum which distributed in northwest Yunnan , were studied through method of rDNA ITS （ internal transcribed spacer ） sequences analysis , and MEGA software .The results showed that there was significant relation between the mycorrhizal fungus of C.yunnanense and that of C.guttatum, in terms of specificity .In another word , mycorrhizal fungus has a obvious tendency in symbiosis with specific Cypripedium.The sequences of mycorrhizal fungus were uploaded to NCBI , and the access numbers were lcl51879, lcl25153, lcl48033, lcl38377, lcl14203, lcl24557, lcl52287 and lcl46937.

关 键 词：云南杓兰 紫点杓兰 菌根真菌 专一性 

分 类 号：S718.81[农业科学—林学]