高效液相色谱法测定热毒平胶囊中大黄素及大黄酚的含量  

DETERMINATION OF EMODIN AND CHRYSOPHANOL IN ANTIET CAPSULE BY HPLC

作　　者：韩峰超[1] 卜跃华 冯雪松 王淑兰[1] 沈君[1] 刘菲[1] 李宏伟 李智勇 

机构地区：[1]沈阳市药品检验所,沈阳110001 [2]中国医科大学制药厂,沈阳110001 [3]沈阳飞龙制药有限公司,沈阳110003

出　　处：《中国药品标准》2001年第1期59-61,共3页Drug Standards of China

摘　　要：用高效液相色谱法测定热毒平胶囊中大黄素和大黄酚含量 结果 线性范围分别为大黄素 0.075～1.50μg(r=0.9999,n=7)、大黄酚 0.106～1.06μg(r=0.9999,n=5),平均回收率分别为大黄素(98.3±1.5)％、大黄酚(101.9±1.2)％。objective: HPLC was used to determine the contents of emodin and chrysophanol in antiet Capsule. Method: The HPLC system consisted of ODS column(150mm×4. 6mm, 10μ) ,methanol -water (98 : 2)as the mobile phase,with detection at 440nm,flow rate 1. 0ml/min. Result: The linear rangs of e-modin and chrysophanol was 0. 075-1.50μg(r = 0. 9999,n = 7) ,0. 106-1. 06μg(r = 0. 9999,n = 5)respec-tively. The mean recovery of emodin and chrysophanol was (98.3±1.5)%, (101. 9±1. 2)%respectively. Conclution: The method is reliable and accurate,useful to quality control.

关 键 词：高效液相色谱法 大黄素 大黄酚 热毒平胶囊 含量 测定 

分 类 号：R286.0[医药卫生—中药学]