导流杂交检测人乳头状瘤病毒感染与宫颈癌临床分期及病理分型关系  

Flow-through hybridization detection of the relationship between HPV infection and cervical cancer clinical staging and pathology typing

作　　者：陈文学[1] 张焜和[1] 邹学森[2] 黄秀珍[2] 陈岳青[2] 

机构地区：[1]南昌大学第一附属医院消化研究所,江西南昌330006 [2]江西省肿瘤医院检验科,江西南昌330029

出　　处：《中华医院感染学杂志》2014年第12期2882-2884,共3页Chinese Journal of Nosocomiology

基　　金：江西省卫生厅基金资助项目(20123105)

摘　　要：目的分析导流杂交检测人乳头状瘤病毒(HPV)感染与宫颈癌临床分期及病理分型的关系,为其筛查和诊断提供依据。方法使用导流杂交技术对医院2009年3月-2012年7月收治的2 176例门诊或住院女性患者,进行HPV检测,对HPV阳性患者进行宫颈组织病理学诊断,分析导流杂交检测结果与病理学诊断结果的关系,所有数据采用SPSS13.0软件进行统计分析。结果 2 176例患者中共检出1 283例HPV阳性,阳性率58.96%,其中1 145例细胞学异常,异常率52.61%,细胞学正常患者HPV阳性率明显低于慢性炎症、宫颈上皮内瘤病变(CIN)及宫颈癌患者,差异有统计学意义(P<0.05);宫颈癌患者HPV阳性率为100.0%,显著高于慢性炎症及CIN患者;1 283例HPV阳性患者病理诊断高危率为40.45%,细胞学正常、慢性炎症及CINⅠ患者高危率明显低于CINⅡ、Ⅲ及宫颈癌患者,差异有统计学意义(P<0.05)。结论 HPV感染是导致宫颈癌发生发展的重要因素,HPV阳性率高、高危分型率高,提示患者宫颈癌风险越高,而宫颈癌导流杂交检测具有操作方便、结果直观、判断准确等优势,可有效提高宫颈癌癌前病变的检测效果。OBJECTIVE To analyze the releationship between HPV infection and cervical cancer clinical staging and pathology typing by using flow-through hybridization detection ,so as to provide the basis for the screening and diagnosis of typing and staging and clinical pathology of cervical cancer . METHODS Using flow-through hybridization technique in our hospital in Mar .2009 to Jul .2012 ,a total of 2176 cases of female outpatients or hospitalization in female patients with HPV were detected in HPV positive patients ,cervical histopathological diagnosis ,analysis of the relationship between the detection results of flow-through hybridization and pathology diagnosis results .SPSS16 .0 software was used for statistical analysis .RESULTS A total of 2176 cases of patients were detected out 1283 cases of HPV positive patients ,the positive rate was 58 .96% ,including 1145 cases of cytological abnormality ,the abnormal rate was 52 .61% .The positive rate of HPV patients was significantly lower than that of normal cytology in patients with chronic inflammation , CIN and cervical cancer and the difference had statistical significance （ P〈 0 .05 ） . The positive rate of HPV cervical carcinoma was 100% , significantly higher than that of chronic inflammation and 1283 cases of CIN ;HPV positive patients with pathological diagnosis of high rate was 40 .42% ,normal cytology ,chronic inflammation and CIN Ⅰ in patients with high risk rate was significantly lower than that of CIN Ⅱ ,CIN Ⅲ and cervical cancer patients and the difference had statistical significance （P〈 0 .05） .CONCLUSIONS HPV infection is an important factor in the development of cervical cancer ,if the positive rate of HPV is high ,the high-risk classification rate will be higher , which is the signal for the higher risk of patients with cervical cancer .Cervical cancer and detection of flow-through hybridization has the advantages of convenient operation , intuitive , accurate judgment and other advantages ,which can effectively improve the detection

关 键 词：导流杂交 人乳头状瘤病毒 感染 宫颈癌 临床分期 病理分型 

分 类 号：R373[医药卫生—病原生物学]