肠道病毒71感染手足口病患儿外周血T淋巴细胞亚群及免疫球蛋白检测研究  

Study on peripheral blood T lymphocytes subsets of children with hand,foot,and mouth disease complicated with enterovirus 71 infection and detection of immunoglobulin

作　　者：袁圣福[1] 李洁[2] 祝伟宏[2] 

机构地区：[1]余姚市人民医院儿科,浙江余姚315400 [2]郴州市第一人民医院儿科,湖南郴州423000

出　　处：《中华医院感染学杂志》2014年第12期3112-3114,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81202535)

摘　　要：目的探讨外周血T淋巴细胞亚群与免疫球蛋白检测在肠道病毒71(EV71)感染手足口病(HFMD)患儿中的临床价值。方法选取47例HFMD患儿作为观察组,另选47名健康儿童作为对照组,分别检测两组小儿外周血T淋巴细胞CD3+、CD4+、CD8+含量及血清IgG、IgA、IgM含量,并在两组之间进行对比分析。结果观察组CD3+、CD4+、CD8+等T淋巴细胞亚群所占比例分别为(54.3±6.4)%、(33.4±4.3)%、(21.4±3.2)%,显著低于对照组的(66.4±6.3)%、(41.2±3.9)%、(25.4±3.5)%,差异有统计学意义(P<0.01);观察组IgA、IgG含量分别为(0.62±0.55)g/L、(7.86±2.64)g/L,均显著低于对照组的(1.17±0.53)g/L、(9.19±2.61)g/L,差异有统计学意义(P<0.05),观察组CD4+/CD8+比值及IgM含量分别为1.60±0.22与(1.23±0.68)g/L,对照组则分别为1.63±0.24与(1.25±0.64)g/L,两组比较差异均无统计学意义。结论 HFMD患儿因EV71感染导致机体免疫功能紊乱,通过外周血T淋巴细胞亚群及血清免疫球蛋白的检测可了解其免疫功能,对于掌握病情及治疗方案的制定等均具有重要的临床价值。OBJECTIVE To explore the clinical value of detection of peripheral blood T lymphocyte subsets or immunoglobulin in diagnosis of enterovirus 71 （EV71） infection in children with hand ,foot ,and mouth disease . METHODS Totally 47 children with hand , foot , and mouth disease were chosen as the observation group , meanwhile 47 health children were set as the control group ,then the contents of the CD3 ＋ ,CD4 ＋ ,and CD8 ＋ in the peripheral blood T lymphocytes subsets and the contents of the serum IgG ,IgA ,and IgM were respectively determined ,and the comparative analysis was performed between the two groups .RESULTS As for the proportion of the children with T lymphocytes subsets , the children with CD3 ＋ accounted for （54 .3 ± 6 .4 ）% in the observation group ,significantly lower than （66 .4 ± 6 .3）% in the control group ;the children with CD4 ＋accounted for （33 .4 ± 4 .3）% in the observation group ,significantly lower than （41 .2 ± 3 .9）% in the control group;the children with CD8 ＋ accounted for （21 .4 ± 3 .2 ）% in the observation group , significantly lower than （25 .4 ± 3 .5）% in the control group ,the differences were significant （P〈0 .01） .The content of IgA of the observation group was （0 .62 ± 0 .55）g/L ,significantly lower than （1 .17 ± 0 .53）g/L of the control group ;the content of IgG of the observation group was （7 .86 ± 2 .64）g/L ,significantly lower than （9 .19 ± 2 .61）g/L of the control group （P〈0 .05） .The ratio of CD4 ＋ /CD8 ＋ was 1 .60 ± 0 .22 in the observation group ,1 .63 ± 0 .24 in the control group;the content of IgM was （1 .23 ± 0 .68）g/L in the observation group ,（1 .25 ± 0 .64）g/L in the control group , the differences were not significant .CONCLUSIONS The EV71 infection may induce the immune dysfunction of the children with hand ,foot ,and mouth disease .It is of significant value in understanding of illness condition and development of treatment programs to detect the peripheral blood T lymphocy

关 键 词：肠道病毒71 手足口病 T淋巴细胞亚群 免疫球蛋白 

分 类 号：R446[医药卫生—诊断学]