HPLC法同时测定2种茶碱类和3种抗癫痫药的血药浓度  

Study on simultaneous analysis of two theophyllines and three antiepileptic drugs in human serum by HPLC

作　　者：陈红君[1] 唐原君[1] 余自成[1] 李静[2] 刘霞[1] 薛冬梅[1] 

机构地区：[1]同济大学附属杨浦医院临床药学与药理学研究室,上海200090 [2]蚌埠医学院

出　　处：《中国临床药学杂志》2014年第3期146-149,共4页Chinese Journal of Clinical Pharmacy

基　　金：同济大学附属杨浦医院"晨光计划"资助项目(编号Ye1201220)

摘　　要：目的建立可同时测定人血清中茶碱、多索茶碱、苯巴比妥、苯妥英钠及卡马西平浓度的HPLC方法。方法色谱柱：XTerra C18（150mm×4．6mm，3．5μm）；流动相：甲醇-水（54：46）；流速0．7mL·min^-1；检测波长205nm；柱温40℃；内标：艾司唑仑。血清样品经3g·L^-1 ZnSO4的甲醇溶液处理后，取20μL直接进样测定。结果茶碱、多索茶碱、苯巴比妥、苯妥英钠及卡马西平分别在质量浓度0．79～101、0．78～100、1．59～204、0．79～101．5、0．47～60mg·L^-1内呈线性关系（r〉0．99），方法日内、日间回收率均为85％～115％，RSD≤13．45％。结论本次建立的同时测定人血清中2种茶碱类与3种抗癫痫药物的HPLC法简便、准确、精密、专属性强，可应用于这类药物的常规血药浓度监测。AIM To establish a HPLC method for simultaneous determination of theophylline, doxofylline, phenobarbital , phenytoin sodium and carbamazepine in human serum. METHODS The serum samples were treated with methanol containing 3 g· L^-1ZnSO4, using estazolam as internal standard. The analytical column was XTerra C18（150mm×4.6mm,3.5μm）and column temperature was 40 ℃. The mobile phase was methanol and water （54：46） . The flow rate was 0.7 mL·min^- 1 and the detection wave length was 205 nm. RESULTS The assay was linear within the range of 0.79 - 101, 0.78 - 100, 1.59 - 204, 0.79 - 101.5 and 0.47 - 60 mg·L^-1 respectively for theophylline, doxofylline, phenobarbital, phenytoin sodium and carbamazepine （ r 〉 0.99）. The recoveries of the analytes were between 85 % - 115% ,and the intra-and inter-day validation were adequate with the RSDs of 13.45% or below. CONCLUSION The method described is convenient, sensitive, accurate and with strong specificity, which will be useful in therapeutic drug monitoring （TDM） of the five analytes.

关 键 词：茶碱 多索茶碱 苯巴比妥 苯妥英钠 卡马西平 HPLC法 血药浓度 

分 类 号：O657.72[理学—分析化学] R742.1[理学—化学]