新型钾离子通道阻滞剂4-氨基吡啶-3-甲醇在大鼠急性脊髓压迫损伤中的作用  

Role of new K＋ channel blocker 4-AP-3-MeOH in acute spinal cord compression injury in rats

作　　者：郑月焕[1] 邹孝军 陈哲[1] 周泽著[1] 施莺莺[2] 王晓宁[2] 曹鹏[1] 

机构地区：[1]上海市伤骨科研究所、上海交通大学医学院附属瑞金医院骨科,200025 [2]上海交通大学医学院附属瑞金医院手术室,200025 [3]山东省日照市莒县人民医院骨三科

出　　处：《中华医学杂志》2014年第19期1503-1506,共4页National Medical Journal of China

基　　金：上海市科委非政府间国际合作科研基金（09410706000）;上海市卫生局重点科研基金（2011018）;上海市卫生局优秀学科带头人培养项目（新百人计划）（XBR2011024）

摘　　要：目的 探讨于大鼠脊髓急性压迫损伤急性期局部应用4-氨基吡啶-3-甲醇（4-AP-3-MeOH）后脊髓神经传导功能改善情况.方法 12只成年雄性SD大鼠随机分为两组：实验组6只、对照组6只,分别于脊髓T11节段压迫损伤后30 min局部应用1 ml 4-AP-3-MeOH（100 μmol/ml）或1ml生理盐水.测定两组压迫损伤前、压迫损伤后30 min、用药干预后体表感觉诱发电位（SSEP）波幅值,并处死动物行脊髓组织LFB染色观察.结果 实验组及对照组压迫损伤前、压迫损伤后30 min及用药干预后SSEP值分别为（1.26 ±0.35）、（0.03 ±0.05）、（0.45 ±0.19） μv,（1.05 ±0.39）、（0.01±0.02）、（0.02±0.02） μv.用药干预后SSEP与压迫损伤后30 min SSEP相比,实验组差异有统计学意义（P＜0.01）,对照组差异无统计学意义（P＞0.05）.脊髓急性压迫损伤后30 min,脊髓大体照可见损伤部位肿胀,出血,劳克坚牢蓝（LFB）染色可见灰质、白质出血水肿,中央管破坏,白质结构破坏、不同程度脱髓鞘病变.结论 脊髓急性压迫损伤后30 min即存在灰白质结构出血、白质脱髓鞘等病理变化,于脊髓损伤局部应用新型钾离子通道阻滞剂4-AP-3-MeOH可显著改善脊髓压迫损伤大鼠SSEP传导,促进损伤脊髓的神经传导功能恢复.Objective To evaluate the effects of 4-aminopyridine-3-methyl hydroxide （4-AP-3-MeOH） in rat＆#39;s acute spinal cord injury.Methods A total of 12 adult male SD rats （250-300 g） were randomly divided into treatment （n =6） and control （n =6） groups.After compressing segment T11 of spinal cord for 30 min,the injured segment received 1 ml 4-AP-3-MeOH （100 μmol/ml） by topically application in treatment group while the control group received 1 ml saline.Somatosensory evoked potential （SSEP） was detected in both groups at pre-injury,30 min post-injury and post-dosing.Then Luxol fast blue （LFB） staining of target spinal segment was performed.Results In treatment group,the values of SSEP at pre-injury,30 min post-injury and post-dosing were 1.26 ± 0.35,0.03 ± 0.05 and 0.45 ± 0.19 μv respectively.Comparing SSEP of 30 min post-injury with post-dosing,the difference was statistically significant （P 〈0.01）.While in control group,the values of SSEP at pre-injury,30 min post-injury and post-dosing were 1.05 ± 0.39,0.01 ± 0.02 and 0.02 ± 0.02 μv respectively.Comparing SSEP of 30 min post-injury with post-dosing,there was no statistical difference （P 〉 0.05）.After 30 min injury,there were swelling and bleeding of spinal cord.LFB staining showed that both gray and white matter had swelling and bleeding and central canal was destroyed with varying degrees of demyelination.Conclusion After 30 min of acute spinal cord injury,there are bleeding of gray and white matter with varying degrees of demyelination.Topical usage of K ＋ blocker 4-AP-3-MeOH can effectively improve the conduction of SSEP after acute spinal cord injury in rats.

关 键 词：脊髓损伤 脊髓压迫症 诱发电位 躯体感觉 4-氨基吡啶-3-甲醇 

分 类 号：R651.2[医药卫生—外科学]