CXCL9和CXCL10在再次移植急性排斥反应中的表达及意义  

Roles of CXCL9 and CXCL10 in acute rejection after retransplantation in mice

作　　者：庄嘉伟 单忠贵[1] 马腾[1] 周小彪[1] 邱水玮[1] 

机构地区：[1]厦门大学附属第一医院心脏外科,361003

出　　处：《中华医学杂志》2014年第20期1584-1586,共3页National Medical Journal of China

基　　金：福建省重点课题（闽科计[2008]59）

摘　　要：目的 探讨趋化因子CXCL9和CXCL10在记忆性T淋巴细胞介导的小鼠心脏移植急性排斥反应中的表达及意义.方法 Balb/c小鼠为供鼠,C57BL/6小鼠为受鼠,进行背部皮肤移植.采用随机数字表法分为3组,每组12只：实验组,C57BL/6小鼠进行皮肤移植饲养6周后,以Balb/c小鼠为供鼠,采用套管法进行颈部异位心脏移植;对照组,C57BL/6小鼠未进行皮肤移植,以Balb/c小鼠为供鼠,直接进行颈部异位心脏移植;同基因移植组,行C57BL/6→C57BL/6颈部异位心脏移植.观察实验组和对照组移植心脏存活时间和组织病理学改变,检测移植物中趋化因子CXCL9和CXCL10基因的相对表达量及CXCL9和CXCL10在受鼠血清中的水平.结果 （1）对照组移植心存活时间为7.75 d,实验组为3.25 d（P ＜0.01）.（2）实验组小鼠手术后外周血趋化因子CXCL9和CXCL10表达明显高于对照组.（3）实验组心脏移植物中CXCL9和CXCL10基因的相对表达量明显高于对照组.（4）对照组移植心脏急性排斥反应的评级为（2.27 ±0.25）级,实验组为（4.12 ±0.03）级,差异有统计学意义（P＜0.01）.结论 血清中趋化因子CXCL9和CXCL10的高表达与急性排斥反应密切相关.Objective To observe the effects of chemokines CXCL9 and CXCL10 on cardiac allograft acute rejection mediated by alloreactive memory T cells in a retransplantation model.Methods Heart transplantation was performed 6 weeks after skin grafting.The mice were divided into 3 groups of control （direct heterotopic heart transplantation without skin grafting） ; experimental （heart transplantation after skin grafting） and syngraft （C57BL/6→C57BL/6,heterotopic heart transplantation） （n =12 each）.Graft survival and the pathological changes of cardiac graft were observed.And related gene expression in cardiac grafts and serum concentration of CXCL9/CXCL10 were detected.Results The mean survival time of control and experimental groups was 7.75 and 3.25 days respectively （P 〈 0.01）.Serum concentrations of CXCL9 and CXCL10 in recipient mice were higher in the experimental group than those in the control group.Compared with the control group,the relative gene expressions of CXCL9 and CXCL10 were higher in the experimental group.According to pathological examinations,the histological rank of cardiac allograft was Grade 2.27 ± 0.25 in the control group versus Grade 4.12 ± 0.03 in the experimental group （P 〈 0.01）.Conclusions CXCL9 and CXCL10 play critical roles in retransplantation mediated by alloreactive memory T cells.And acute rejection of cardiac allograft is more extensive in retransplantation.

关 键 词：趋化因子类 T淋巴细胞 心脏移植 急性排斥反应 再次移植 

分 类 号：R654.2[医药卫生—外科学]