肺炎支原体BALB/c小鼠支气管肺泡灌洗液中IFN-γ、IL-4、IL-5、IL-10表达和气道阻力的变化  

The alteration of IFN-γ,IL-4,IL-5 and IL-10 expressions in bronchoalveolar lavage fluid and airway resistance in mycoplasma pneumoniae infected BALB/c mice

作　　者：张晗[1] 焦绪勇[1] 尚云晓[1] 王莉[1] 何梦博[2] 韩晓华[1] 王桂珍[2] 

机构地区：[1]中国医科大学,附属盛京医院小儿呼吸内科,辽宁沈阳110004 [2]中国医科大学,基础医学院病原生物学教研室,辽宁沈阳110005

出　　处：《解剖科学进展》2014年第3期240-245,共6页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(No.2013021020)

摘　　要：目的通过建立肺炎支原体(MP)感染BALB/c小鼠的模型,探讨MP感染后小鼠支气管肺泡灌洗液(BALF)中炎性因子(IFN-γ和IL-4、IL-5和IL-10)的含量变化及其对气道阻力的影响。方法 50只BALB/c小鼠随机分为MP感染组(MP组)和PBS对照组(N组),每组25只,分别于接种后第3d、7d、14d、21d和30d从各组中取5只鼠,取肺脏制作病理切片,HE染色观察病理组织学变化;RT-PCR对模型进行鉴定;ELISA试剂盒检测BALF中IFN-γ、IL-4、IL-5和IL-10含量;同时用肺功能仪测定各组小鼠肺的吸气阻力、呼气阻力和顺应性。结果 MP感染小鼠后IFN-γ、L-4、IL-5和IL-10的含量较N组明显升高(<0.05);吸气阻力、呼气阻力较N组增加(<0.05),顺应性降低较N组增加(<0.05),第7天时达高峰,随后下降30天恢复正常。结论 MP感染BALB/c小鼠后气道阻力增加、顺应性降低,炎性因子过表达。Objective To establish mycoplasma pneumoniae（MP） infected BALB/c mice model to explore the expression of inflammatory cytokines （IFN-γ and IL-4, IL-5 and IL-10） in bronchoalveolar lavage fluid（BALF） and the change of airway resistance in MP BALB/c mice. Methods 50 BALB/c mice were randomly divided into： MP infection group and PBS control group averagely. HE staining and RT-PCR were used to identify the histological changes and the model respectively, ELISA kit to detect the expression of IFN-γ, IL-4, IL-5 and IL-10; the inspiratory resistance, expiratory resistance and compliance were determined with spirometer. Results The expression levels of IFN-γ, L-4, IL-5 and IL-10 in bronchoalveolar lavage fluid were significantly higher in MP infected mice than in PBS control mice（ P 〈0.05）; the inspiratory resistance and expiratory resistance increased, but compliance decreased in MP infected mice than in PBS control mice（P 〈0.05）, reached peak 7 days and then declined to normal 30 days after infection. Conclusion The airway inflammatory factors overexpressed in BALF, airway resistance increased, compliance decreased in MP infected BALB/c mice.

关 键 词：肺炎支原体 BALB C小鼠 炎性因子 气道阻力 

分 类 号：R562.2[医药卫生—呼吸系统]