1-甲基-4-苯基-1,2,3,6-四氢吡啶对食蟹猴嗅球多巴胺能神经元的影响  

Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP

作　　者：程炜[1] 任振华[1,2] 关云谦[2] 吴迪[2] 岳峰 李光武[1] 

机构地区：[1]安徽医科大学神经生物学教研室,合肥230032 [2]首都医科大学宣武医院细胞生物室,北京100053 [3]广西南宁灵康赛诺科生物科技有限公司,南宁530000

出　　处：《解剖学报》2014年第3期333-337,共5页Acta Anatomica Sinica

基　　金：安徽省科学自然基金资助项目(1308085MH119)

摘　　要：目的建立食蟹猴1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病系统性模型,探讨嗅球中多巴胺(DA)及多巴胺/cAMP调节磷蛋白(DARPP32)的表达情况。方法 3只成年健康食蟹猴,静脉注射MPTP,建立帕金森病系统性模型,取出嗅球,切片,免疫组织化学染色DA和DARPP32,摄片并观察DA和DARPP32在食蟹猴嗅球中的分布及表达情况,采用Image Pro-Plus软件,半定量分析模型组和正常组之间DA和DARPP32的表达差异。结果食蟹猴嗅球中DA和DARPP32神经元集中分布于突触小球层,DA神经纤维分布于突触小球层,而DARPP32神经纤维分布于嗅球各层,以突触小球层(GL)和外网状层(EPL)最为密集。MPTP损伤后,与正常对照组比较,DA和DARPP32神经元及神经纤维均减少,以DA神经元及神经纤维减少明显。结论食蟹猴嗅球中DA神经元和神经纤维分布于突触小球层。食蟹猴MPTP帕金森病系统性模型的嗅球DA能神经元和纤维明显减少,可能与帕金森病嗅觉障碍有关。Objective To explore the localization and expression of dopaminergic neurons in olfactory bulb of cynomolgus monkeys damaged by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP）.Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of dopamine neurons （ MPTP group ） and three adult cynomolgus monkeys were as a control group .Immunohistochemical staining was performed to examine the localization and expression of dopaminergic neurons in the olfactory bulb in normal and MPTP group monkeys .The numbers of DA-positive and DARPP32-positive cells were counted and the average absorbance was measured in normal and MPTP group .Results DA and DARPP32 positive neurons were concentrated in the glomerular layer （ GL） of olfactory bulb.DA positive nerve fibers were distributed in the GL while DARPP 32 positive nerve fibers appeared in all layers , and most nerve fibers were in GL and external plexiform layers （EPL）.After MPTP injury, compared with the normal control group , DA and DARPP32 positive neurons and nerve fibers decreased in MPTP group and DA neurons and nerve fibers decreased significantly . Conclusions DA neurons and nerve fibers are in the GL of cynomolgus monkey olfactory bulb .DA neurons and fibers are significantly reduced in the olfactory bulb of cynomolgus monkeys damaged by MPTP , which may be associated with the dysosmia in Parkinson ’ s disease .

关 键 词：1-甲基-4-苯基-1 2 3 6-四氢吡啶 嗅球 多巴胺 多巴胺cAMP调节磷蛋白 免疫组织化学 食蟹猴 

分 类 号：R743.1[医药卫生—神经病学与精神病学]