基于ITS条形码序列的刺桐姬小蜂分子鉴定  

Application of DNA barcoding technology to the identification of Quadrastichus erythrinae Kim (Hymenoptera:Eulophidae)

作　　者：黄蓬英[1] 林玲玲[1] 李雯琳[2] 洪钦阳[2] 张玲华[2] 廖富荣[1] 

机构地区：[1]厦门出入境检验检疫局,厦门361026 [2]福建农林大学植物保护学院,福州350003

出　　处：《应用昆虫学报》2014年第3期749-756,共8页Chinese Journal of Applied Entomology

基　　金："十二五"国家科技支撑计划课题(2012BAK11B03);厦门市科技计划项目(3502Z20112016)

摘　　要：【目的】刺桐姬小蜂Quadrastichus erythrinae Kim体型小,传统的形态学鉴定方法难以快速准确识别。【方法】本研究测定了刺桐姬小蜂的rDNA ITS1和ITS2序列,根据18S rDNA部分序列,利用MEGA的最大相似法(Maximum Likehood)构建系统发育树。根据刺桐姬小蜂ITS1和ITS2序列设计了特异引物,应用特异引物对单只刺桐姬小蜂进行PCR扩增,可稳定地扩增出明显的目的DNA条带。【结果】研究表明,基于ITS基因的DNA条形码技术可以用于刺桐姬小蜂的快速准确鉴定。【结论】因此,采用ITS1和ITS2区的特异性引物可对刺桐姬小蜂进行快速分子鉴定。[Objectives] Quadrastichus erythrinae Kim （Hymenoptera： Eulophidae） is too small to be identified quickly and accurately by traditional morphological methods. [Methods] The complete sequences of the rDNA ITS1 and ITS2 genes were determined for Q. erythrinae. A phylogenetic tree was established on the basis of variation in 18SrDNA part sequences using MEGA Maximum Likelihood. Species-specific primers of Q. erythrinae were designed based on the rDNA ITS1 and ITS2 sequences. The results of PCR amplification of rDNA ITS1 and ITS2 indicate that these species-specific primers could reliably identify this species. [Results] The results suggest that the species-specific primers developed produce clear, unique and reproducible target DNA bands from individual adult wasps. Molecular identification of Q. erythrinae using species-specific primers from the rDNA ITS1 and ITS2 region is therefore feasible. [Conclusion] This study identified a possible avenue for the molecular identification of Q erythrinae,

关 键 词：刺桐姬小蜂 ITS基因 DNA条形码 特异引物 分子鉴定 

分 类 号：S433[农业科学—农业昆虫与害虫防治]