含非甲基化二核苷酸的寡脱氧核苷酸1826对大鼠放射性肺纤维化的预防作用  

Protection of CpG ODN 1826 against radiation pulmonary fibrosis in rats

作　　者：李轩[1] 乔田奎[1] 庄喜兵[1] 张继红[1] 

机构地区：[1]复旦大学附属金山医院肿瘤科,上海200540

出　　处：《中华放射医学与防护杂志》2014年第5期334-337,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：上海市卫生局重点课题基金（2012-4014）

摘　　要：目的 初步探讨含非甲基化二核苷酸的寡脱氧核苷酸（CpG ODN） 1826对大鼠放射性肺纤维化的防护作用.方法 采用6MVX射线单次左肺照射20 Gy,建立大鼠放射性肺纤维化模型.SD大鼠按完全随机法分为健康对照组、单纯照射组和药物干预组3组,每组30只.在照后0、1、3、5和7d,腹腔注射CpG ODN1826.照后5、15、30和90 d,取材,记录肺系数.照射肺石蜡切片HE染色、Masson染色,行肺纤维化评分.ELISA法检测血清TGF-β1浓度,碱化水解法检测肺组织羟脯氨酸（Hyp）浓度.结果 成功建立了放射性肺纤维大鼠模型.照后5d,健康对照组肺系数低于单纯照射组和药物干预组（t =3.046、2.252,P＜0.05）.照后15、30和90 d,药物干预组肺系数低于单纯照射组（t=4.120、5.226和5.719,P＜0.05）.单纯照射组及药物干预组照后30 d肺系数最高.药物干预组肺纤维化评分低于单纯照射组（t=3.212、4.959,P＜0.05）.药物干预组血清TGF-β1浓度低于照射组（t=4.138、5.924、5.294和4.076,P＜0.05）.单纯照射组照射肺Hyp含量高于药物干预组（t=7.527、8.416,P＜0.05）.结论 CpG ODN1826可以降低放射性肺纤维化大鼠血清TGF-β1浓度及Hyp含量,预防放射性肺纤维化.Objective To explore the protectional function of CpG ODN 1826 against radiation pulmonary fibrosis in rats.Methods The rat left lung was exposed to 20 Gy of 6 MV X-rays for establishing a radiation pulmonary fibrosis model.SD rats were randomly divided into control group,irradiated group and intervention group,with 30 rats in each group.CpG ODN 1826 was intraperitoneally injected into rats at 0,1,2,5 and 7 d post-irradiation.The rats were terminated at 5,15,30 and 90 d post-irradiation,and the lung indexes were recorded.Paraffin sections of the radiated lung were conducted with HE staining and Masson staining,the pulmonary fibrosis scores were recorded.The serum concentrations of TGF-β1 and hydroxyproline （Hyp） were measured.Results The radiation pulmonary fibrosis rat model was successfully established.The lung indexes of the control group were lower than those of the irradiated and intervention groups at 5 d post-irradiation （t =3.046,2.252,P ＜ 0.05）.The lung indexes of the intervention group were lower than those of the irradiated group （t =4.120,5.226,5.719,P ＜ 0.05）.Pulmonary fibrosis scores of intervention group were lower than those of irradiated group （t =3.212,4.959,P ＜ 0.05）.The serum concentrations of TGF-β1 of irradiated group were higher than those of the intervention group （t =4.138,5.924,4.138,5.924,P ＜ 0.05）.The Hyp in the lung of irradiated group was higher than that of intervention group （t =7.527,8.416,P ＜ 0.05）.Conclusions CpG ODN1826 will not worse the radiation pulmonary fibrosis,on the contrary,it could reduce the serum concentrations of TGF-β1 and the lung content of Hyp in radiation pulmonary fibrosis,and protects rat against radiation pulmonary fibrosis.

关 键 词：含非甲基化二核苷酸的寡脱氧核苷酸1826 放射性肺纤维化 辐射防护 

分 类 号：R142[医药卫生—公共卫生与预防医学]