Src家族调节Nav1.1在耳蜗螺旋神经节神经元的表达  

Src family kinases affect the expression of Nav1.1 in spiral ganglion neurons

作　　者：曾庆娇[1] 陈慧英[1] 苏纪平[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁530021

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第11期789-792,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金(No:81060082;81360157);广西自然科学基金(No:2011GXNSFA018249)

摘　　要：目的:研究蛋白酪氨酸激酶Src家族对耳蜗螺旋神经节神经元的电压门控钠离子通道基因和蛋白表达的影响。方法:采用RT-PCR和Western blot技术分别观察在Src家族抑制剂的作用下,Nav1.1mRNA和蛋白的表达变化情况。结果:Src家族抑制剂PP2(10μmol/L)和SU6656(2μmol/L)作用后,螺旋神经节神经元Nav1.1mRNA的表达量分别减少至26%±0.8%和36%±1.5%(P<0.05),蛋白的表达量分别减少至39%±12.5%和53%±1.7%(P<0.05)。结论:抑制蛋白酪氨酸激酶Src家族可下调Nav1.1mRNA和蛋白在螺旋神经节神经元的表达水平。Objective：To investigated the effects of Src family kinases on the expression of mRNA and protein of Nav1. 1 in spiral ganglion neurons. Method：RT-PCR and Western blot techniques respectively explored the level of expression of mRNA and protein of Nav1. 1 in spiral ganglion neurons by Src family kinases inhibitor. Result： An application of the inhibitor of Src family kinases which was PP2（ 10μmol/L）and SU6656（2μmol/L）gived rise to the mRNA decreasing to 26%± 0.8 % and 36 % ±1.5% respectively （P〈0.05）, and protein reducing to 39% ± 2.5 % and 53% ±1.7% severally（P〈 0. 05 ）. Conclusion： Administration of the inhibitor of Sre family kinases could decrease the expression of mRNA and protein,of Nav1. 1 in spiral ganglion neurons.

关 键 词：螺旋神经节神经元 NAV1 1 Src家族 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]