同型半胱氨酸对人外周血内皮祖细胞功能的影响  

Effects of Homocysteine on Function of Human Peripheral Blood Progenitor Cells

作　　者：娄晓盈[1] 张泉[1] 曹露[1] 牟娜娜[1] 谭红梅[1] 

机构地区：[1]中山大学中山医学院病理生理教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2014年第3期329-333,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81370371)

摘　　要：【目的】探讨同型半胱氨酸(Hcy)对外周血内皮祖细胞(EPC)迁移、粘附能力的影响。【方法】密度梯度离心法获取单个核细胞,接种于铺有人纤维连接蛋白包被的培养皿,培养7 d后免疫荧光鉴定EPC。实验分对照组(Control,CT)、50μmol/L腺苷(adenosine,Ade)组(A50)、50μmol/L Hcy组(H50)、50μmol/L Hcy+50μmol/L Ade组(A50+H50)和50μmol/L Hcy+50μmol/L Ade+10 ng/mL血管内皮生长因子(VEGF)组(A50+H50+VEGF),不同给药组处理后检测内皮祖细胞迁移、粘附功能。各项实验都至少重复3次。【结果】Dil-acLDL(红色)和FITC-lectin(绿色)染色双阳性的细胞可鉴定为EPC。与CT组相比,Hcy、Ade可抑制EPC细胞的迁移(P<0.05);Hcy、Ade还可抑制EPC细胞粘附到纤维连接蛋白及脐静脉内皮细胞(HUVEC)(P<0.05)。与Hcy、Ade组相比,Hcy联合Ade可进一步显著抑制EPC细胞的迁移和粘附功能(P<0.05)。加入VEGF可显著增加EPC细胞的迁移及粘附功能(P<0.05)。【结论】Hcy可显著抑制EPC的迁移粘附能力,加入VEGF后可明显改善这种抑制效应。[Objective] This study investigated the effect of moderate concentration of homocysteine （Hcy） on the migration and adhesion function of human endothelial progenitor cells （EPC）.[Method] Total mononuclear cells were isolated from human peripheral blood by Ficoll density gradient centrifugation.The cells were plated on fibronectin-coated dishes,and then cultured in EGM-2 medium.After 7 days culture,the attached cells were identified by immunofluorescence.EPC were divided into control group （CT）,50 μmol/L adenosine group （A50）,50 μmol/L Hcy group （H50）,50 μmol/L Hcy＋50 μmol/L Ade group （A50＋H50） and 50 μmol/L Hcy＋ 50 μmol/L Ade ＋ 10 ng/mL VEGF group （A50＋H50＋VEGF）.EPC migration and adhesion were detected.All data were from at least three independent experiments.[Result] EPC were characterized as double positive for Dil-acLDL and FITC-lectin.The migration and adhesion of EPC were significantly attenuated in H50 and A50 group compared with the CT group （P ＜ 0.05）.Hcy combined with Ade further inhibited EPC migration and adhesion compared with H50 and A50 group （P ＜ 0.05）.The supplement of recombinant VEGF promoted EPC migration and adhesion （P ＜ 0.05）.[Conclusion] Hcy can significantly inhibit EPC migration and adhesion,which was abolished by the supplement of recombinant VEGF.

关 键 词：同型半胱氨酸 内皮祖细胞 细胞迁移 细胞粘附 

分 类 号：R54[医药卫生—心血管疾病]