外源性硫化氢通过抗氧化应激逆转过氧化氢诱导的人脐静脉内皮细胞衰老  

Hydrogen Sulfide Reverses Human Umbilical Vein Endothelial Cells Senescence Induced by Hydrogen Peroxide

作　　者：牛海名[1] 宋志明[1] 彭沛[1] 刘勇[1] 郝宝顺[1] 朱洁明[1] 陈璘[1] 钱孝贤[1] 

机构地区：[1]中山大学附属第三医院心血管内科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2014年第3期346-351,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金项目(81370447);广东省科技计划项目(2011B031800024);广州市科技计划项目(2011B031800155)

摘　　要：【目的】探讨硫化氢(H2S)对过氧化氢(H2O2)诱导的人脐静脉内皮细胞衰老的影响及其抗氧化机制。【方法】建立H2O2诱导的人脐静脉内皮细胞衰老模型,采用不同浓度的硫氢化钠(NaHS)预处理内皮细胞,观察其对衰老的作用。【结果】与对照组相比,60μmol/L H2O2预处理后,能显著提高细胞SAβ-gal阳性率和血浆纤溶酶原激活物抑制剂-1(PAI-1)的表达,同时增加丙二醛(MDA)含量,但减少过氧化物歧化酶1(SOD1)的表达。而NaHS的预处理可以显著减少细胞SAβ-gal阳性率和PAI-1的表达,减少MDA的含量,增加SOD1的表达。【结论】NaHS可以逆转H2O2诱导的内皮细胞衰老,其机制与抗氧化应激有关。[Objective] To investigate the effect of hydrogen sulfide （H2S） on human umbilical vein endothelial cells （HUVEC） senescence established by hydrogen peroxide （H2O2）.[Methods] The senescence was established by H2O2 and the effects of NaHS on the senescence was investigated.[Results] Compared with the control group,after treated with 60 μmol/L H2O2,the number of SA β-gal positive cells and the expression of PAI-1 significantly increased.Meantime,MDA concentration increased but the expression of SOD1 decreased in the senescent cells.However,after pretreatment of NaHS,the ratio of SA β-gal positive cells,the expression of PAI-1 and the MDA concentration decreased significantly,but the expression of SOD1 increased.[Conclusion] NaHS could reverse HUVEC senescence induced by H2O2.The anti-peroxide effects of NaHS appears to be particular importance.

关 键 词：硫化氢 内皮细胞 过氧化氢 衰老 氧化应激 

分 类 号：R339.38[医药卫生—人体生理学]