Effect of pyrite, elemental sulfur and ferrous ions on EPS production by metal sulfide bioleaching microbes  

黄铁矿、单质硫、亚铁离子对浸矿微生物产生胞外多聚物的影响(英文)

作　　者：贺治国[1,2] 杨彦平[1,2] 周珊[1,2] 胡岳华[1,2] 钟慧[3] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]中南大学生物冶金教育部重点实验室,长沙410083 [3]中南大学生命科学学院,长沙410083

出　　处：《Transactions of Nonferrous Metals Society of China》2014年第4期1171-1178,共8页中国有色金属学报（英文版）

摘　　要：Extracellular polymeric substances （EPS） produced by acidophilic bioleaching microorganisms play an important role in the production of acid mine drainage and metal sulfide bioleaching. EPS mediate the contact between microbial cells and growth substrates, having a pivotal role in organic film formation and bacterium-substratum interactions. The production and chemical composition of EPS produced by seven bioleaching strains grown with different substrates were studied. Analysis of the EPS extracted from these strains indicated that the EPS consisted of carbohydrates, proteins and galacturonic acid. The contents of EPS, carbohydrates, proteins and galacturonic acid of EPS were largely related to the kind of strain used and culture condition. The results show that EPS productions of microbes grown with pyrite were significantly higher than those of microbes grown with sulfur or FeSO4·7H2O. The highest EPS production of the seven acidiphilic strains was （159.43±3.93） mg/g, which was produced by Leptospirillum ferriphilum CBCBSUCSU208015 when cultivated with pyrite.嗜酸浸矿微生物产生的胞外多聚物(EPS)在酸性矿坑水的产生和硫化矿的浸出过程中,有着非常重要的影响,胞外多聚物(EPS)介导细胞与能源物质的接触,对有机薄膜的形成和细菌与基础物之间的相互作用起着重要的作用。对7株浸矿菌在不同能源培养物下产生的EPS的量以及EPS的化学成分进行研究,发现EPS含有化学成分糖、蛋白质、糖醛酸等,细菌的种类和能源物质对EPS的量和成分有很大影响。结果表明,以黄铜矿为能源物质的细菌产生的EPS要比以单质硫和亚铁为能源物质产生的EPS量多,EPS含量最高为(159.43±3.93)mg/g,是由Leptospirillum ferriphilum CBCBSUCSU208015在黄铁矿为能源物质下产生的。

关 键 词：acid mine drainage carbohydrates extracellular polymeric substances galacturonic acid metal sulfide BIOLEACHING 

分 类 号：TD925.5[矿业工程—选矿]