利用大配子体构建马尾松遗传图谱  

Construction of Genetic Linkage Map in Pinus Massoniona Using Megagametophytes

作　　者：何卫龙[1] 张逢凯 潘婷[1] 季孔庶[1] 

机构地区：[1]林木遗传与生物技术省部共建教育部重点实验室、南京林业大学,南京210037

出　　处：《分子植物育种》2014年第3期421-431,共11页Molecular Plant Breeding

基　　金：林业公益性行业科研专项经费(201104010);国家“十二五”科技支撑项目(2012BAD 01B02);江苏高校优势学科建设工程项目(PAPD)共同资助

摘　　要：本研究以单株马尾松的142个大配子体为作图群体,利用筛选的55对SSR引物和142对SRAP引物获得502个标记,构建了一张包含237个标记、18个连锁群的马尾松遗传连锁图谱。马尾松连锁图谱总图距为1 462.1 cM,最大连锁群图距为136.4 cM,最小连锁群图距为36.7 cM,平均每个连锁群长度为81.2 cM,标记之间平均图距为6.2 cM。此外,利用L16(45)正交试验表对影响PCR结果的Mg2+、dNTP、模板DNA、引物浓度、Taq DNA聚合酶进行试验,建立了适合马尾松SSR-PCR和SRAP-PCR的反应体系。In this study, 142 gametophytes from one tree ofP/nus massortiona were used as mapping population. 55 SSR primers and 142 SRAP primers （the two kinds of primers contained total 502 markers） were used as markers. The experiment constructed a P. massoniona genetic linkage map which contained 237 markers and 18 linkage groups. The total map length was 1 462.1 cM and the map distance of the largest linkage group was 136.4 cM while the smallest linkage group was 36.7 cM. The average length of linkage groups was 81.2 cM and the average map distance between markers was 6.2 cM. In addition, L16（4^5） orthogonal test was designed to optimize Mg2＋, dNTP, template DNA, primer concentration, Taq DNA polymerase which could affect the PCR result, a suitable reaction system for P. massoniana SSR-PCR and SRAP-PCR was established.

关 键 词：马尾松 SSR SRAP 体系优化 遗传连锁图谱 

分 类 号：S791.248[农业科学—林木遗传育种]