^(125)I标记重组融合蛋白dTMP-GH在小鼠体内分布的实验研究  

Experimental research on distribution of ^(125)I labeled recombinant fusion protein dTMP-GH in mice

作　　者：申明强[1] 陈默[1] 王崧[1] 粟永萍[1] 王军平[1] 

机构地区：[1]第三军医大学预防医学院全军复合伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆市纳米医药工程技术研究中心,重庆400038

出　　处：《局解手术学杂志》2014年第3期221-224,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(31071025);广东省科技计划项目(2012A080202001)

摘　　要：目的研究重组融合蛋白dTMP-GH在小鼠体内的分布情况,明确其是否具有靶向分布特点。方法实验室制备dTMP-GH重组融合蛋白;用放射性125I标记融合蛋白dTMP-GH后,按100μg/kg的标准小鼠尾静脉注射125I-dTMP-GH,分别于给药后5 min、15 min、30 min、1 h、2 h、4 h、8 h、12 h、24 h取心、肝、脾、肾、股骨、甲状腺等进行放射性计数。结果制备得到纯度大于98%的重组融合蛋白dTMP-GH;125I标记率为71.53%,放化纯度为96.53%,比活度为0.22 MBq/μl;尾静脉注射125I标记的dTMPGH后30 min股骨的放射性计数占到注射总量的10%,并随时间推移经肝脏和肾脏代谢。结论融合蛋白dTMP-GH经尾静脉注射后在小鼠体内主要分布于骨髓,具有骨髓组织偏向分布特点。Objective To assess the distribution of recombinant fusion protein dTMP-GH in mice and to determine whether it is of tar- geted distribution characteristics. Methods A laboratory scale preparation of dTMP-GH recombinant fusion protein was obtained. Protein dT- MP-GH was labeled with radioactive 125I,then mice were sacrificed at 5 min,15 min,30 min,1 h,2 h,4 h,8 h,12 h,24 h after tail vein injec- tion of 125 I-dTMP-GH at a dose of 100 μg/kg, and the organs and tissues （heart, liver, spleen, kidney, bone and thyroid） were collected for radioactive counting. Results Preparation of purified （ 〉 98% ） dTMP-G was obtained. 125I labeling rate was 71.53%, radiochemical purity was 96.53% ,and specific activity was 0.22 MBq/μl. 30 min after tail vein injection of 125I labeled dTMP-GH, radioactivity accounted for 10% of the total injected in femoral, and metabolism was carried via liver and kidney over time. Conclusion Fusion protein mainly distribu- ted in bone marrow via tail vein injection in mice, which expressed that dTMP-GH has the characteristics of selective distribution in bone mar- row tissue.

关 键 词：125I标记 dTMP-GH 血小板 小鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R558.2[医药卫生—基础医学]