小叶榕叶抗炎成分分析及活性评价  

Chemical analysis and activity evaluation of anti-inflammatory constituents of Ficus microcarpa L. f.

作　　者：黄洋[1] 邵慧凯[1] 李康[1] 刘盛权 谢淑桐 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《中成药》2014年第6期1227-1233,共7页Chinese Traditional Patent Medicine

基　　金：教育部留学回国人员科研启动基金([2011]1139);国家自然科学基金青年基金资助项目(30801515)

摘　　要：目的对小叶榕叶抗炎有效部位的化学成分进行分离鉴定和活性筛选。方法采用硅胶柱层析对小叶榕叶水提液进行分离纯化,利用IR、1H-NMR、13C-NMR对其结构进行鉴定。Griess法分析各化合物和巨噬细胞RAW264.7共同培养液中NO量;采用ELISA法检测培养液中TNF-α表达。结果得到10个化合物分别是苯甲酸(1)、对羟基苯丙酸(2)、3,5-二甲氧基-4-羟基苯甲酸(3)、对羟基苯乙酸(4)、水杨酸(5)、3,5-二甲氧基-4-羟基-苯乙酮(6)、邻羟基苯丙酸(7)、十五烷酸(8)、2-羟基-3-甲基丁酸(9)、对羟基苯甲酸仲丁酯(10)。分离到的化合物2、4、5、7、9、10系首次从该植物中得到。化合物1、2、3、4、5、10对NO因子的释放抑制作用显著。而化合物1、3、4、5、6、7对TNF-α因子表达抑制较为明显。结论药效试验发现小叶榕叶发挥抗炎药效的作用可能是多成分、多指标协同作用的结果。AIM To carry out the separation, analysis of chemical constituents of anti-inflammatory effective fraction from Ficus microcarpa L. f and their bioactivity screening. METHODS The separation and purification of the water extraction of Ficus microcarpa L.f. were performed on silica gel column and the structure analyses of constituents were completed by IR, 1H-NMR, 13 C-NMR. Griess reaction was used for assaying the nitric oxide （NO） level in culture fluid of macrophage RAW264.7 and constituents obtained , ELISA for TNF-α level. RESULTS Ten compounds were prepared and identified as benzoic acid （1）, p-hydroxybenzene propanoic acid （2）, syringic acid （3）, 4-hydroxyphenylacetic acid （4）, salicylic acid （5）, acetosyringonecas （6）, 3- （2-hydroxyph-enyl） propanoic acid （ 7 ）, pentadecylicacid （ 8 ）, 2-hydroxy-3-methylbutyricacid （ 9 ）, sec-butyl-4-Hydroxybenzoate （10）. The compounds 2, 4, 5, 7, 9, 10 were isolated from Ficus microcarpa L.f. for the first time. The com- pounds 1, 2, 3, 4, 5, 10 could significantly inhibit the release of NO. However, the compounds 1, 3, 4, 5, 6, 7 could markedly downregulate the expression of TNF-α. CONCLUSION The experiment confirms that the pharmaceutical effect of anti-inflammatory constituents from Ficus microcarpa L.f. could be comprehensive and synergistic.

关 键 词：小叶榕叶 化学成分 分离纯化 鉴定 活性评价 

分 类 号：R284.1[医药卫生—中药学]