补骨脂有效成分在大鼠血清、肝、肾中的测定  

Determination of effective constituents in Psoralea Fructus contained in serum,liver and kidney of rats

作　　者：张昀[1] 张瑜[1] 

机构地区：[1]河南大学药学院,河南开封475001

出　　处：《中成药》2014年第6期1237-1240,共4页Chinese Traditional Patent Medicine

基　　金：河南省教育厅研究项目(14B35009)

摘　　要：目的建立大鼠血清、肝脏、肾脏中补骨脂素、异补骨脂素定量测定的方法。方法采用乙酸乙醋-正己烷（8：1）为萃取溶剂进行样品处理，采用HPLC法测定，色谱柱AgilentTC—C18（2）（4．6mm×250mm，5μm）；以氯霉素为内标物，流动相为水-乙腈，梯度洗脱，体积流量1mL／min，柱温30℃，检测波长295nm（0—10min，氯霉素），246nm（10～25min，补骨脂素、异补骨脂素）。结果补骨脂素、异补骨脂素在0．1～10．0mg／L范围内，在血清、肝匀浆液、肾匀浆液中均能达到很好的分离。结论本方法简便准确，可同时测定给药后大鼠血清、肝脏和肾脏中补骨脂素和异补骨脂素。AIM To establish a quantitative HPLC method for determining the contents of psoralen and isopso- ralen in rat＇ s serum, liver and kidney. METHODS The samples were extracted by ethyl acetate and n-butanol （8 ： 1 ）. The HPLC method was performed on the Agilent TC-C18 （2） and the mobile phase was water-aeetonitrile using chloramphenicol as the internal standard in the flow rate of 1.0 mL/min. The detection wavelength was set at 295 nm （ 0-10 min） for chloramphenieol, 246 nm （10-25 min） for psoralen and isopsoralen, the column temper- ature was 30 ℃. RESULTS The two components showed good linear relationship in the range of 0. 1-10. 0 mg/ mL, and could be separated in serum, liver and kidney of rats. CONCLUSION This method can be used to sim- ultaneously determine the content of psoralen and isopsoralen in serum, liver and kidney of rats.

关 键 词：补骨脂 补骨脂素 异补骨脂素 高效液相色谱 大鼠 

分 类 号：R284.1[医药卫生—中药学]