人血红蛋白α、β基因以可溶形式在大肠杆菌中的表达  

SOLUBLE EXPRESSION OF α AND β GENES OF HUMAN HEMOGLOBIN IN ESCHERICHIA COLI

作　　者：张浩[1] 王全立[2] 毛秉智[1] 冯启[2] 杜芝燕[2] 刘晓达[2] 李晓霞[3] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]军事医学科学院野战输血研究所 [3]解放军总医院检验科

出　　处：《解放军医学杂志》2001年第3期207-209,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：将人血红蛋白α、β珠蛋白基因分别克隆进 pET 2 1b原核表达载体中 ,宿主菌经IPTG化学诱导 ,在1.6× 10 5处有特异的蛋白带表达。表达产物以可溶形式存在 ,α珠蛋白的表达量达总菌体蛋白的 5 % ,β珠蛋白的表达量达 15 % ,并经Western Blotting杂交证实。Fragments of α and β genes were cloned into expression plasmid pET-21b. Bacteria harbouring expression plasmid was induced with IPTG,and a band of expressed protein of 16 kD was found in PAGE. They expressed as soluble products. α expression product reached about 5% of total bacterial protein,β expression product reached about 15% of total bacterial protein. The products were confirmed by Western-blotting.

关 键 词：珠蛋白 大肠杆菌 基因表达 血红蛋白A 血红蛋白β 

分 类 号：R331.141[医药卫生—人体生理学]