HPLC同时测定舒乐洗剂中苦参碱和小檗碱  

HPLC Simultaneous Determination of Matrine and Berberine in Shule Lotion

作　　者：王慧[1,2] 毛春芹 周渊[1] 吴晓燕[1] 郎永英[1] 陆兔林 

机构地区：[1]南京中医药大学药学院,南京210023 [2]徐州市中医院药剂科,江苏徐州221003

出　　处：《中国实验方剂学杂志》2014年第12期88-90,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：徐州市科技局社会发展项目(XM12B048)

摘　　要：目的：建立HPLC同时测定舒乐洗剂中苦参碱和小檗碱的含量测定方法。方法：采用Kromasil C_18色谱柱（4．6mm×250mm，5μm），以甲醇-乙腈-0．1％磷酸溶液（每100mL含十二烷基磺酸钠0．1g）为流动相进行梯度洗脱，流速1．0mL·min-1，柱温30℃，检测波长230nm。结果：苦参碱和小檗碱的色谱峰面积与质量呈良好的线性关系，线性范围分别为0．4305～8．61μg（r=1．0000），0．00585～0．1171μg（r=0．9999）；平均回收率分别为98．9％，99．1％。结论：该方法简便、准确、重复性好，可用于舒乐洗剂的质量控制。Objective： To establish a quantitative determination method for matrine and berberine in Shule lotion. Method： The determination was performed on Kromasi] Clscolumn （4.6 mm ×250 mm, 5 μm） with mobile phase consisting of methanol, acetonitrile and 0. 1% phosphoric acid solution in gradient elution. The flow rate was 1.0 mL ·min-1, the column temperature was 30 ℃ , and the detection wavelength was 230 nm. Result： The method had good linear relationship for matrine and berberine in the ranges of 0. 430 5-8.61 μg （r = 1. 000 0） ,0. 005 85-0. 117 1 μg （r = 0. 999 9）, respectively. The average recoveries was 98.9% for matrine, 99.1% for berberine. Conclusion： The method is simple, accurate and specific. It provides a reliable way for evaluating the quality of Shule lotion.

关 键 词：舒乐洗剂 苦参碱 小檗碱 

分 类 号：R284.1[医药卫生—中药学]