伊达比星脂质体的制备及其包封率的测定  

Preparation of idarubicin liposome and its entrapment efficiency determination

作　　者：王玲玲[1] 邓盛齐[1] 尹婕[1] 任静[1] 

机构地区：[1]成都大学,四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2014年第6期447-450,共4页Chinese Journal of Antibiotics

摘　　要：目的制备伊达比星脂质体，建立伊达比星脂质体包封率的测定方法。方法采用薄膜分散．超声合并挤压法制备伊达比星脂质体，通过葡聚糖凝胶柱色谱分离游离药物和脂质体，用HPLCN定伊达比星含量，计算包封率。结果伊达比星脂质体平均包封率为65．42％，载药量为7．70％。凝胶柱色谱柱回收率99．76％，伊达比星在0．05-30．0μg／mL范围内线性关系良好（r=0．9999）。结论薄膜分散-超声合并挤压法适用于制备伊达比星脂质体，分析方法准确可靠可以用于伊达比星脂质体包封率的测定。Objective To prepare idarubicin liposome and establish a method for determination of its entrapment efficiency. Methods The liposome was prepared by film dispersion-ultrasonic combined extrusion technique. The liposome and free idarubicin were separated by Sephadex-G50 chromatography. The concentration of idarubicin in the liposome was detected by HPLC, then the entrapment efficiency was evaluated. Results The mean entrapment efficiency of idarubicin liposome was 65.42% and the drug loading was 7.70%. The recovery rate was 99.76%. The standard curve was linear over the range of 0.05-30.0μg/mL with the correlation of 0.9999. Conclusion The film dispersion-ultrasonic combined extrusion technique is suitable for preparing idarubicin liposomes. This HPLC method was accurate and sensitive, and can be applied to the determination of idarubicin liposomes.

关 键 词：伊达比星 薄膜分散超声合并挤压法 脂质体 包封率 

分 类 号：R978.19[医药卫生—药品]