GC-MS定性定量分析生物检材中的安眠酮  

QUALITATIVE AND QUANTITATIVE ANALYSIS OF METHAQUALONE IN BIOMATERIAL USING GC-MS

作　　者：曾浩天 刘晓云 李文美[3,4] 陈园园 黎雪萍 彭运平 王继华 

机构地区：[1]广州市公安局交警支队,广州510640 [2]广州正孚检测技术有限公司,广州510663 [3]广州万孚生物技术有限公司,广州510663 [4]华南理工大学,广州510640

出　　处：《中国药物依赖性杂志》2014年第3期196-200,共5页Chinese Journal of Drug Dependence

摘　　要：目的:建立定性定量检测人体尿液、血液及唾液中安眠酮的分析方法。方法:尿液、血液、唾液等生物样本中的安眠酮经固相萃取法进行提取后,用气相色谱-质谱仪进行定性定量分析,从线性、抗干扰性、精密度、回收率及稳定性等方面对方法进行验证。结果:安眠酮浓度在0-400.0 ng·mL-1的范围内线性良好(r≥0.999),方法的最低检出量为8.0 ng·mL-1;最低定量限为100.0 ng·mL-1;方法的日内、日间精密度CV≤5%(n=6);各基质中前处理回收率在±10.0%(n=3)范围内。结论:本方法检测准确、灵敏、特异性好,可满足尿液、血液、唾液等生物检材中安眠酮的定性定量分析。Objective ： methaqualone in biomaterial To develope a method for the qualitative and quantitative analysis of （human urine, blood and saliva）. Methods： Biomaterial samples were extracted with SPE extraction, then determinated by GC/MS. In the validation test, linearity, interference, accuracy, recovery percent and stability of extracted samples were tested. Resu./.ts_： The linearity was established in biomaterial between 0 and 400.0 ng· mL^-1, with correlation coefficients higher than 0.999 ; The limit of detection was 8.0 ng· mL^-1 ; the limit of quantitation was 100.0 ng· mL^-1 ; the intra -day precision and inter -day precision of the assays CV was lower than 5% （n = 6） ; the recovery percent was between - 10.0% and 10.0% （ n = 3 ）. Conclusion ： The method was precise, accurate, sensitive and specific. It is an effective method for the qualitative and quantitative analysis of methaqualone in biomaterial.

关 键 词：生物检材 安眠酮 固相萃取 GC—MS 

分 类 号：R991[医药卫生—毒理学]